Product Details
| Alternative Name: | Apo-2L, TNFSF 10, CD253 |
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| MW: | 23 kDa |
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| Source: | Produced in E. coli. Soluble mouse TRAIL (aa 118-291). |
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| UniProt ID: | P50591 |
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| Formulation: | Liquid. In PBS containing 0.1mM DTT. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Endotoxin Content: | <0.1 EU/µg TRAIL, determined using chromogenic Limulus amebocyte lysate (LAL) test. |
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| Biological Activity: | Induces apoptosis at 0.1 and 1.0µg/ml, in primary cultured mouse hepatocytes, in the presence of cycloheximide (5µg/ml) or actinomycin D (0.5µg/ml). Induces apoptosis in Jurkat cells (a TRAIL-sensitive human cell line) at 1µg/ml, within 3 hours. Inhibits secondary clonal expansion of mouse CD8+ T cells in vitro (50ng/ml and greater). |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | Ligand for TRAIL receptor (DR4, DR5). |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE Analysis: Lane 1: MW Marker, Lane 2: 2 µg TRAIL.
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Product Literature References
Intranasal Administration of a TRAIL Neutralizing Monoclonal Antibody Adsorbed in PLGA Nanoparticles and NLC Nanosystems: An In Vivo Study on a Mouse Model of Alzheimer’s Disease: T. Musumeci, et al.; Biomedicines
10, 985 (2022),
Abstract;
K63-linked ubiquitination regulates RIPK1 kinase activity to prevent cell death during embryogenesis and inflammation: Y. Tang, et al.; Nat. Commun.
10, 4157 (2019),
Abstract;
N-glycosylation of mouse TRAIL-R restrains TRAIL-induced apoptosis: Y. Estornes, et al.; Cell Death Dis.
9, 494 (2018),
Abstract;
Full Text
Targeting Immune Privilege to Prevent Pathogenic Neovascularization: J. Roychoudhury, et al.; Invest. Ophthalmol. Vis. Sci.
51, 3560 (2010),
Application(s): Intravitreal injections in mice for studies on choroidal neovascularization,
Abstract;
Full Text
Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells: A. Lundqvist, et al.; Blood
113, 6120 (2009),
Application(s): Death induction of sensitized Renca cells,
Abstract;
Full Text
IRF-5 Is a Mediator of the Death Receptor-induced Apoptotic Signaling Pathway: G. Hu & B.J. Barnes; J. Biol. Chem.
284, 2767 (2009),
Application(s): Death induction of HCT116 cells,
Abstract;
Full Text
Treating Metastatic Solid Tumors With Bortezomib and a Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor Agonist Antibody: A. Shanker, et al.; J. Natl. Cancer Inst.
100, 649 (2008),
Application(s): Death induction of sensitized Renca, TBJ, 4T1 cells,
Abstract;
Full Text
The role of NF-κB-1 and NF-κB-2-mediated resistance to apoptosis in lymphomas: L. Bernal-Mizrachi, et al.; PNAS
103, 9220 (2006),
Application(s): Death induction of 204-3-1, NK-H and NK-92 cells and analysis of resistance to TRAIL-induced cell death of Daudi, SC, F8 and BLA cells,
Abstract;
Full Text
Keratins Modulate c-Flip/Extracellular Signal-Regulated Kinase 1 and 2 Antiapoptotic Signaling in Simple Epithelial Cells: S. Gilbert, et al.; Mol. Cell Biol.
24, 7072 (2004),
Application(s): Death induction of sensitized mouse primary hepatocytes,
Abstract;
Full Text
TRAIL and KILLER Are Expressed and Induce Apoptosis in the Murine Preimplantation Embryo: J.K. Riley, et al.; Biol. Reprod.
71, 871 (2004),
Application(s): Death induction of sensitized mice blastocysts,
Abstract;
Full Text
The proteasome inhibitor PS-341 sensitizes neoplastic cells to TRAIL-mediated apoptosis by reducing levels of c-FLIP: T.J. Sayers, et al.; Blood
102, 303 (2003),
Application(s): Death induction of sensitized C1498 and Renca cells and sensitized C1498-bone marrow cocultures in mice,
Abstract;
Full Text
Roles of TNF-Related Apoptosis-Inducing Ligand in Experimental Autoimmune Encephalomyelitis: B. Hilliard, et al.; J. Immunol.
15, 166 (2001),
Application(s): Death induction of L929 cells,
Abstract;
Full Text
