| Alternative Name: | Tyrosine protein kinase Lyn B |
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| MW: | ~85 kDa |
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| Source: | Produced in insect cells. Full length active enzyme. Produced in a baculovirus expression system. |
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| UniProt ID: | P07948-2 |
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| Formulation: | Liquid. 0.1 mg/ml in 50 mM TRIS-HCl, pH 7.5, containing 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Specific Activity: | 180 nmol/min/mg. Kinase activity was measured as the molar amount of phosphate incorporated into poly (Glu-Tyr) at 30°C using a concentration of 50μM [32P]ATP in 25mM MOPS, pH 7.2, 12.5mM β-glycerophosphate, 5mM EGTA, 2mM EDTA, 25mM MgCl2, 0.25mM DTT (added right before using). |
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| Application Notes: | Useful for kinetic and functional studies, phosphorylation of target substrates, drug screening. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | After initial defrost, aliquot product into individual tubes and refreeze the remaining, unused enzyme quickly by snap-freezing in a dry/ice ethanol bath or liquid nitrogen, if possible. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Replacement for ADI-PPK-431 |
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| Regulatory Status: | RUO - Research Use Only |
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