| Alternative Name: | Cell division cycle 25C, Dual specificity phosphatase CDC25C |
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| MW: | 54 kDa |
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| Source: | Produced in E. coli. Full length human CDC25C. |
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| UniProt ID: | P30307 |
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| Formulation: | Liquid. In 50mM TRIS/HCl, pH 8.0, 150mM NaCl, 5mM DTT, 0.1mM EDTA, 0.03% Brij 35, 20% glycerol. |
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| Purity Detail: | Partially purified by single-step affinity chromatography and gel filtration. |
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| Specific Activity: | ≥14 U/mg assayed by 3-O-methyl fluorescein phosphate (OMFP) hydrolysis at pH 8.2, 30°C. One unit is equal to 1 nmole phosphate hydrolyzed from OMFP per minute. |
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| Application Notes: | Useful for kinetic studies and high throughput screening. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | CDC25 protein tyrosine phosphatases activate cyclin dependent kinases by dephosphorylating them, promoting cell division. All three of the genes encoding the CDC25 phosphatases are considered potential oncogenes and the enzymes are drug discovery targets. CDC25C activates CDC2/cyclin B which controls entry into mitosis. CDC25C, itself, is regulated by phosphorylation and binding to 14-3-3 proteins. |
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| Regulatory Status: | RUO - Research Use Only |
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