Alternative Name: | Cell division cycle 25A, Dual specificity phosphatase CDC25A |
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MW: | 55kDa |
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Source: | Produced in E. coli. Full length human CDC25A. |
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UniProt ID: | P30304 |
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Formulation: | Liquid. In 50mM TRIS-HCl, pH 8.0, containing 150mM sodium chloride, 5mM DTT, 0.1mM EDTA, 0.03% Brij 35 and 20% glycerol. |
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Purity: | ≥80% (SDS-PAGE) |
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Purity Detail: | Purified by multi-step chromatography. |
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Specific Activity: | ≥150 U/µg assayed by 3-O-methyl fluorescein phosphate (OMFP) hydrolysis at pH 8.2, 30°C. One unit is equal to 1 nmole phosphate hydrolyzed from OMFP per minute. |
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Application Notes: | Useful for kinetic studies and high throughput screening. |
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Shipping: | Dry Ice |
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Handling: | After opening, prepare aliquots, freeze in liquid nitrogen and store at -80°C. Avoid freeze/thaw cycles. |
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Regulatory Status: | RUO - Research Use Only |
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