The peptide consists of two domains - an antennapedia homeodomain that confers cell permeability and the T735 to E745 region of IKKβ which is the NEMO binding domain (NBD). It is a selective inhibitor of NFκB activation. Blocks interaction of NEMO (an IKB complex regulatory protein) with the IκB kinase complex. The peptide (100 µM) inhibits E-selectin expression in human umbilical vein endothelial cells treated with TNFα and inhibits LPS-induced nitric acid release from a macrophage cell line In an experimental mouse model of acute inflammation, IKK-NBD peptide (topically applied) inhibited PMA induced ear inflammation. Sold under license from Vanderbilt University
Product Details
| Sequence: | Asp-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Thr-Ala-Leu-Asp-Trp-Ser-Trp-Leu-Gln-Thr-Glu |
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| MW: | 3694.0 |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | Lyophilized solid |
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| Solubility: | Soluble in DMSO (20mM). Note: This peptide will precipitate when added to tissue culture medium, even to 100µM. However, enough will remain in solution to enter many cell types. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Ethanol Decreases Inflammatory Response in Human Lung Epithelial Cells by Inhibiting the Canonical NF-kB-Pathway: K. Mors, et al.; Cell. Physiol. Biochem.
43, 17 (2017),
Abstract;
Full Text
Selective inhibition of NF-kappaB activation by a peptide that blocks the interaction of NEMO with the IkappaB kinase complex: M. J. May et al.; Science
289, 5484 (2000),
Abstract;
Identification of Grb2 as a novel binding partner of tumor necrosis factor (TNF) receptor I: E. Hildt et al.; J. Exp. Med.
189, 1707 (1999),
Abstract;
Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation: S. Yamaoka et al.; Cell
93, 1231 (1998),
Abstract;
