Sensitive fluorogenic substrate for TACE (TNFα converting enzyme, ADAM17). Also cleaved by ADAM10 and likely other ADAMs and MMPs. The kcat/Km of TACE for this substrate is 7-fold higher than for the same peptide with the Mca-Dpa fluorophore-quencher pair. FAM fluorescence is quenched by the TAMRA group until cleavage separates them. Ex.: 485nm, Em.: 535nm, although the following Ex/Em can also be used: 490,494/515,520nm. This substrate is useful for inhibitor screening, kinetic analysis, and cellular activity assay.
Product Details
| Alternative Name: | ADAM17 substrate II (fluorogenic) |
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| Sequence: | 5-FAM-Ser-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Lys(5-TAMRA)-NH2 |
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| MW: | 2157.3 |
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| Formulation: | Lyophilized. |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | Solid. |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Handling: | Protect from light and moisture. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Ectodomain Shedding of RAGE and TLR4 as a Negative Feedback Regulation in High-Mobility Group Box 1-Activated Aortic Endothelial Cells: W.S. Yang, et al.; Cell. Physiol. Biochem.
51, 1632 (2018),
Abstract;
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