Sensitive fluorogenic substrate for cathepsin S and cathepsin L. Also cleaved by cathepsin B, cathepsin V, kallikrein 8, and factor VIIa/TF but not cathepsin K nor kallikrein 2. Ex.: 380 nm, Em.: 460 nm, although the following Ex/Em can also be used: 355,375/440,450. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144).
Product Details
| Alternative Name: | Cathepsin S & L substrate (fluorogenic) |
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| Sequence: | Z-Val-Val-Arg-AMC . HCl [Z=Cbz=Benzyloxycarbonyl; AMC=7-amino-4-methylcoumarin] |
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| MW: | 663.8 |
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| CAS: | 124485-41-2 |
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| Purity: | ≥99% (TLC) |
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| Appearance: | Lyophilized solid. |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Handling: | Protect from light. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Cell penetrable, clickable and tagless activity-based probe of human cathepsin L: D. Dana, et al.; Bioorg. Chem.
85, 505 (2019),
Application(s): Activity assay,
Abstract;
The lysosome system is severely impaired in a cellular model of neurodegeneration induced by HSV-1 and oxidative stress: H. Kristen, et al.; Neurobiol. Aging
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Cloning and characterization of a basic cysteine-like protease (cathepsin L1) expressed in the gut of larval Diaprepes abbreviatus L.(Coleoptera: Curculionidae): S. Ben-Mahmoud, et al.; J. Insect Physiol.
72, 1 (2015),
Abstract;
Ascorbic acid modulation of iron homeostasis and lysosomal function in trabecular meshwork cells: P. Xu, et al.; J. Ocul. Pharmacol. Ther.
30, 246 (2014),
Abstract;
Full Text
Development of a highly potent, selective, and cell-active Inhibitor of cysteine cathepsin L-A hybrid design approach: D. Dana, et al.; Chem. Commun. (Camb.)
50, 10875 (2014),
Abstract;
Cathepsin B is up-regulated and mediates extracellular matrix degradation in trabecular meshwork cells following phagocytic challenge: K. Porter, et al.; PLoS One
8, e68668 (2013),
Abstract;
Full Text
