Fluorogenic substrate for angiotensin converting enzyme-1 (ACE1) N-domain. The somatic form of ACE1 is a zinc-dependent peptidyl dipeptidase comprising two catalytic domains referred to as the N- and C-domains. The testicular form, due to a downstream start site, contains only the C-domain. Abz fluorescence is quenched by the Dnp group until cleavage (at Asp-Lys) separates them. Ex: 320 nm, Em: 420 nm. This quenched substrate is useful for assay of cell culture ACE1 N-domain activity, inhibitor screening and kinetic analysis.
Product Details
| Alternative Name: | ACE1 (N-domain) substrate (fluorogenic) |
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| Sequence: | Abz-Ser-Asp-Lys(Dnp)-Pro-OH[Abz=o-aminobenzoicacid;Dnp=2,4-dinitrophenyl] |
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| MW: | 730.9 |
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| Purity: | ≥97% |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Divergence in the activity of the N- and C- catalytic domains of ACE1 - implications for the role of the renin-angiotensin system in Alzheimer's disease: N.S. Al Mulhim, et al.; Acta Neuropathol. Commun.
7, 57 (2019),
Abstract;
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