Fluorogenic substrate specific for cathepsin K, among other cathepsin family members. This substrate is also cleaved by granzyme A, thrombin, tryptase, trypsin, and kallikreins. Ex.: 365 nm, Em.: 440 nm, although the following Ex/Em can also be used: 355,380/450,460. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144).
Product Details
| Alternative Name: | Z-Gly-Pro-Arg-AMC |
| |
| Sequence: | Z-Gly-Pro-Arg-AMC . HCl [Z=Cbz=Benzyloxycarbonyl; AMC=7-amino-4-methylcoumarin] |
| |
| MW: | 656.1 |
| |
| Formulation: | C31H37N7O7 |
| |
| Purity: | ≥97% (HPLC) |
| |
| Appearance: | White powder. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
A Pilot Study to Assess the Circulating Renin-Angiotensin-System in COVID-19 Acute Respiratory Failure: D.C. Files, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol.
321, L213 (2021),
Abstract;
Cell penetrable, clickable and tagless activity-based probe of human cathepsin L: D. Dana, et al.; Bioorg. Chem.
85, 505 (2019),
Application(s): Activity assay,
Abstract;
The lysosome system is severely impaired in a cellular model of neurodegeneration induced by HSV-1 and oxidative stress: H. Kristen, et al.; Neurobiol. Aging
68, 5 (2018),
Abstract;
Effect of tryptase inhibition on joint inflammation: a pharmacological and lentivirus-mediated gene transfer study: A. Denadai-Souza, et al.; Arthritis Res. Ther.
19, 124 (2017),
Abstract;
Full Text
Upconversion nanocarriers encapsulated with photoactivatable Ru complexes for near‐infrared light‐regulated enzyme activity: Z. Chen, et al.; Small
13, (2017),
Abstract;
Cloning and characterization of a basic cysteine-like protease (cathepsin L1) expressed in the gut of larval Diaprepes abbreviatus L.(Coleoptera: Curculionidae): S. Ben-Mahmoud, et al.; J. Insect Physiol.
72, 1 (2015),
Abstract;
Approaches to design non-covalent inhibitors for human granzyme B (hGrB): M. Kim, et al.; Org. Biomol. Chem.
12, 8952 (2014),
Abstract;
Ascorbic acid modulation of iron homeostasis and lysosomal function in trabecular meshwork cells: P. Xu, et al.; J. Ocul. Pharmacol. Ther.
30, 246 (2014),
Abstract;
Full Text
Development of a highly potent, selective, and cell-active Inhibitor of cysteine cathepsin L-A hybrid design approach: D. Dana, et al.; Chem. Commun. (Camb.)
50, 10875 (2014),
Abstract;
Cathepsin B is up-regulated and mediates extracellular matrix degradation in trabecular meshwork cells following phagocytic challenge: K. Porter, et al.; PLoS One
8, e68668 (2013),
Abstract;
Full Text
Lysosomal basification and decreased autophagic flux in oxidatively stressed trabecular meshwork cells: implications for glaucoma pathogenesis: K. Porter, et al.; Autophagy
9, 581 (2013),
Abstract;
Full Text
