Fluorogenic substrate for several matrix metalloproteinases (MMPs). It is cleaved by MMP-1, MMP-3, MMP-7, MMP-8, MMP-9, MMP-11, MMP-12, MMP-13, and ADAM9/MDC9, but only slightly by MMP-2 and not at all by ADAM17/TACE. Ex/Em= 340nm/440nm, although 365/450 is also appropriate. Highly quenched, with strong fluorescence by Nma at 440nm once cleavage separates it from the aromatic Dnp moiety. Compatible with most fluorometers.
Product Details
| Alternative Name: | Matrix metalloproteinase substrate, Dnp-Pro-β-cyclohexyl-Ala-Gly-Cys(Me)-His-Ala-Lys(Nma)-NH2 |
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| Sequence: | Dnp-P(Cha)G(CMe)HA(KNma)-amide |
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| MW: | 1077.4 |
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| Purity: | ≥98% (HPLC) |
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| Appearance: | lyophylized solid |
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| Solubility: | Soluble in DMSO (at least 20mM). |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Handling: | Protect from light. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Chemical Composition and Effect against Skin Alterations of Bioactive Extracts Obtained by the Hydrodistillation of Eucalyptus globulus Leaves: P. Moreira, et al.; Pharmaceutics
14, 561 (2022),
Abstract;
Parenchymal border macrophages regulate the flow dynamics of the cerebrospinal fluid: A. Drieu, et al.; Nature
611, 585 (2022),
Abstract;
Polyphenol-Rich Extracts Obtained from Winemaking Waste Streams as Natural Ingredients with Cosmeceutical Potential: M.S. Matos, et al.; Antioxidants
8, 355 (2019),
Application(s): Substrate in MMP-1 activity assays.,
Abstract;
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Targeting mechanotransduction mechanisms and tissue weakening signals in the human amniotic membrane: D.W. Barrett, et al.; Sci. Rep.
9, 6718 (2019),
Abstract;
Full Text
Nucleic acid-induced potentiation of matrix metalloproteinase-9 enzymatic activity: T. Duellman, et al.; Biochem. J.
475, 1597 (2018),
Abstract;
Full Text
Toll-Like Receptor 4-Independent Carbon Tetrachloride-Induced Fibrosis and Lipopolysaccharide-Induced Acute Liver Injury in Mice: Role of Hepatic Stellate Cells: S. Kumar, et al.; Am. J. Pathol.
187, 1356 (2017),
Abstract;
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The tumor necrosis factor-alpha converting enzyme (TACE): a unique metalloproteinase with highly defined substrate selectivity: M.J. Mohan, et al.; Biochemistry
41, 9462 (2002),
Abstract;
N-Aryl sulfonyl homocysteine hydroxamate inhibitors of matrix metalloproteinases: further probing of the S(1), S(1)’, and S(2)’ pockets: S. Hanessian, et al.; J. Med. Chem.
44, 3066 (2001),
Abstract;
Metalloprotease-disintegrin MDC9: intracellular maturation and catalytic activity: M. Roghani, et al.; J. Biol. Chem.
274, 3531 (1999),
Abstract;
Detection system for reaction-rate analysis in a low-volume proteinase-inhibition assay: W.P. Ambrose, et al.; Anal. Biochem.
263, 150 (1998),
Abstract;
Evaluation of fluorometric and zymographic methods as activity assays for stromelysins and gelatinases.: A.R. Quesada, et al.; Clin. Exp. Metastasis
15, 26 (1997),
Abstract;
A high throughput fluorogenic substrate for interstitial collagenase (MMP-1) and gelatinase (MMP-9): D.M. Bicket, et al.; Anal. Biochem.
212, 58 (1993),
Abstract;
