Replaces Prod. #: ALX-380-068
Trichostatin A (TSA) is a potent and reversible inhibitor of histone deacetylases. In HeLa cells, TSA blocked cell cycle progression at G1 and induced a 12-fold increase in intracellular levels of gelsolin. In cells latently infected with HIV-1, TSA induced the transcriptional activation of the HIV-1 promoter, which resulted in a marked increase in virus production. In NIH 3T3 cells, TSA induced reversion of oncogenic ras-transformed cells to a normal morphology. In Jurkat cells, TSA inhibited IL-2 gene expression (IC50=73nM) and displayed immunosuppressive activity in a mouse model.
Induces increased acetylation of GATA4, a cardiac-specific transcription factor and increases cardiac muscle cell differentiation. Trichostatin A is a useful tool for induction of hyperacetylation of cellular histones and for further elucidation of their role in gene expression. Induces cell growth arrest at both G1 and G2/M phases. may induce apoptosis in some cases. It can induce accelerated dedifferentiation of primordial germ cells into embryonic germ cells.
Product Details
| Alternative Name: | [R-(E,E)]-7-[4-(Dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide |
| |
| Formula: | C17H22N2O3 |
| |
| MW: | 302.4 |
| |
| Source: | Isolated from Streptomyces platensis. |
| |
| CAS: | 58880-19-6 |
| |
| Purity: | ≥98% (HPLC) |
| |
| Appearance: | Off-white to beige solid. |
| |
| MeltingPoint: | 150°C |
| |
| Solubility: | Soluble in DMSO, dimethyl formamide, acetonitrile, methanol or 100% ethanol |
| |
| Shipping: | Ambient Temperature |
| |
| Long Term Storage: | -20°C |
| |
| Use/Stability: | Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Please mouse over
Product Literature References
Design, Synthesis, and biological evaluation of HDAC6 inhibitors based on Cap modification strategy: X. Li, et al.; Bioorg. Chem.
125, 105874 (2022),
Abstract;
Structural mass spectrometry decodes domain interaction and dynamics of the full-length Human Histone Deacetylase 2: Z. Soloviev, et al.; Biochim. Biophys. Acta Proteins Proteom.
1870, 140759 (2022),
Abstract;
Identification of novel pathways and molecules able to down-regulate PHOX2B gene expression by in vitro drug screening approaches in neuroblastoma cells: E. Di Zanni, et al.; Exp. Cell Res.
336, 43 (2015),
Application(s): Cell Culture,
Abstract;
Inhibitors of histone deacetylases: correlation between isoform specificity and reactivation of HIV type 1 (HIV-1) from latently infected cells: K. Huber, et al.; J. Biol. Chem.
286, 22211 (2011),
Abstract;
Full Text
Chromatin remodeling agent trichostatin A: a key-factor in the hepatic differentiation of human mesenchymal stem cells derived of adult bone marrow: S. Snykers et al.; BMC Dev. Biol.
7, 24 (2007),
Abstract;
Trichostatin A induces myocardial differentiation of monkey ES cells: M. Hosseinkhani et al.; Biochem. Biophys. Res. Commun.
356, 386 (2007),
Abstract;
The NAD+ precursor nicotinic acid improves genomic integrity in human peripheral blood mononuclear cells after X-irradiation: K. Weidele, et al.; DNA Repair
52, 12 (20017),
Abstract;
Trichostatin A is a histone deacetylase inhibitor with potent antitumor activity against breast cancer in vivo: D.M. Vigushin; Clin. Cancer Res.
7, 971 (2001),
Abstract;
Histone deacetylase inhibitors: inducers of differentiation or apoptosis of transformed cells: P.A. Marks et al.; J. Natl. Cancer Inst.
92, 1210 (2000),
Abstract;
Regulation of the human O(6)-methylguanine-DNA methyltransferase gene by transcriptional coactivators cAMP response element-binding protein-binding protein and p300: K.K. Bhakat & S. Mitra; J. Biol. Chem.
275, 34197 (2000),
Abstract;
Effects of cell density and trichostatin A on the expression of HDAC1 and p57Kip2 in Hep 3B cells: S.G. Gray et al.; Biochem. Biophys. Res. Commun.
245, 423 (1998),
Abstract;
Retinoblastoma protein recruits histone deacetylase to repress transcription: A. Behm et al.; Nature
391, 597 (1998),
Abstract;
A histone deacetylase inhibitor potentiates retinoid receptor action in embryonal carcinoma cells: S. Miucci et al.; PNAS
94, 11295 (1997),
Abstract;
Histone deacetylase inhibitor activates the WAF1/Cip1 gene promoter through the Sp1 sites: Y. Sowa et al.; Biochem. Biophys. Res. Commun.
241, 142 (1997),
Abstract;
Induction of caspase-3 protease activity and apoptosis by butyrate and trichostatin A (inhibitors of histone deacetylase): dependence on protein synthesis and synergy with a mitochondrial/cytochrome c-dependent pathway: V. Medina et al.; Cancer Res.
57, 3697 (1997),
Abstract;
A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p: J. Taunton et al; Science
272, 408 (1996),
Abstract;
Selective inhibition of IL-2 gene expression by trichostatin A, a potent inhibitor of mammalian histone deacetylase: I. Takahashi et al.; J. Antibiot. (Tokyo)
49, 453 (1996),
Abstract;
Transcriptional activation and chromatin remodeling of the HIV-1 promoter in response to histone acetylation: C. Van Lint et al.; EMBO J.
15, 1112 (1996),
Abstract;
Trichostatin A and trapoxin: novel chemical probes for the role of histone acetylation in chromatin structure and function: M. Yoshida et al.; Bioessays
17, 423 (1995),
Abstract;
Trichostatin A inhibits both ras-induced neurite outgrowth of PC12 cells and morphological transformation of NIH3T3 cells: M. Futamura et al.; Oncogene
10, 1119 (1995),
Abstract;
Trichostatin A induces morphological changes and gelsolin expression by inhibiting histone deacetylase in human carcinoma cell lines: Y. Hoshikawa et al.; Exp. Cell Res.,
214, 189 (1994),
Abstract;
Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A: M. Yoshida et al.; J. Biol. Chem.
265, 17174 (1990),
Abstract;
A new antifungal antibiotic, trichostatin: N. Tsuji et al.; J. Antibiot. (Tokyo)
29, 1 (1976),
Abstract;
