The set contains 5 ready-to-use reagents that induce apoptosis through different mechanisms. Actinomycin D, an antineoplastic antibiotic, inhibits RNA synthesis. Camptothecin, an inhibitor of nuclear topoisomerase, induces apoptosis in many types of cells. Cycloheximide, an active antibiotic against many yeast and fungi, inhibits protein synthesis. Dexamethasone, an active and highly stable glucocorticoid, probably induces apoptosis by binding and activating the intracellular glucocorticoid receptor. Etoposide, a derivative of podophyllotoxin, inhibits topoisomerase activity.
Product Details
| Recommended Dilutions/Conditions: | We recommend using 1,000x dilutions for inducing apoptosis in cell cultures. Optimal doses may vary for different cells and culture conditions and must be determined individually. |
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| Contents: | Contains 5 ready-to-use reagents:
- Actinomycin D, 50µl (10mM), violet cap
- Camptothecin, 1ml (5.7mM), red cap
- Cycloheximide, 1ml (100mM), green cap
- Dexamethasone, 1ml (10mM), blue cap
- Etoposide, 100µl (100mM), yellow cap |
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| Formulation: | Liquid. In DMSO |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl: S.J. Martin, et al.; J. Exp. Med.
182, 1545 (1995),
Abstract;
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature
376, 37 (1995),
Abstract;
Dexamethasone and etoposide induce apoptosis in rat thymocytes from different phases of the cell cycle: H.O. Fearnhead, et al.; Biochem. Pharmacol.
48, 1073 (1994),
Abstract;
Topoisomerase inhibitors induce apoptosis in thymocytes: Y. Onishi, et al.; Biochim. Biophys. Acta
1175, 147 (1993),
Abstract;
