- One-step, ready-to-use solution with no radioisotopes
- High sensitivity that correlates with the [3H]-thymidine incorporation assay
- High-throughput screening without a solubilization step
- More sensitive and stable than MTT, MTS or WST-1
The Cell Counting Kit-8 is a colorimetric assay kit used to measure cell proliferation and cytotoxicity.
It is a ready-to-use solution that does not require radioisotopes and correlates with the [3H]-thymidine incorporation assay. It can be added directly to the cell media for fast, high-throughput screening without a solubilization process obtaining highly reproducible and accurate results. CCK-8 has shown to achieve higher sensitivity and stability than MTT, MTS or WST-1.
Cell Counting kit-8 performance
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation.
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation. CCK-8 shows greatest sensitivity.
CCK-8 sensitivity for HeLa and HL60 cells are more sensitive than MTT
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Product Details
| Alternative Name: | CCKi-8 |
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| Applications: | Colorimetric detection
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| Quantity: | 100 tests: 1 mL
500 tests: 5 mL
2500 tests: 5 x 5 mL |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Contents: | WST-8 solution, 1-methoxy-PMS |
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| Technical Info/Product Notes: | Principle: Employs the tetrazolium salt WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium . monosodium salt), that produces a highly water soluble formazan dye upon biochemical reduction in the presence of an electron carrier, 1-methoxy-PMS. The amount of the yellow colored formazan dye generated by dehydrogenases in cells is directly proportional to the number of viable cells in a culture medium. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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