Product Details
Clone: | MEM-28 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Human thymocytes and T lymphocytes. |
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UniProt ID: | P08575 |
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Species reactivity: | Human
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Specificity: | Recognizes all isoforms of CD45. |
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Applications: | Flow Cytometry, ICC, IHC (PS), IP, WB
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Recommended Dilutions/Conditions: | Flow Cytometry (1µg/ml) Immunocytochemistry (10µg/ml, paraformaldehyde fixation can be used) Immunohistochemistry (paraffin sections, no pre-treatment of tissue sections is essential) Western Blot (1µg/ml; Positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate. Sample preparation: buffer with laurylmaltoside, 2x non-reducing SDS. Application note: non-reducing conditions. SDS-PAGE (6% separating gel)) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein A-affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.4, containing 15mM sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice Not Frozen |
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Long Term Storage: | +4°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
PTEN is a protein phosphatase that targets active PTK6 and inhibits PTK6 oncogenic signaling in prostate cancer: D.J. Wozniak, et al.; Nat. Commun.
8, 1508 (2017),
Abstract;
Full Text
Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules: L. Cermak, et al.; J. Biol. Chem.
277, 7955 (2002),
Abstract;
Full Text
Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74): V. Horejsi, et al.; Folia Biol (Praha)
34, 23 (1988),
Abstract;
Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen: V. Horejsi, et al.; Folia Biol (Praha)
32, 12 (1986),
Abstract;