Replaces Prod. #: ALX-804-840/1
Product Details
| Alternative Name: | Interleukin-33, IL-1F11, NF-HEV |
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| Clone: | Nessy-1 |
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| Host: | Mouse |
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| Isotype: | IgG1κ |
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| Immunogen: | Recombinant human IL-33 (aa 112-270) (Prod. No. ALX-522-098). |
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| UniProt ID: | O95760 |
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| Gene/Protein Identifier: | 90865 (Entrez GeneID), 608678 (OMIM) |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
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| Specificity: | Recognizes pro-IL-33 (precursor) and mature IL-33. |
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| Applications: | ELISA, IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Immunoprecipitation (1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | Cited samples:
For an overview on cited samples please click here. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Immunohistochemical staining of endogenous human IL-33 in different human tissues (paraffin sections) using MAb to IL-33 (Nessy-1) (Prod. No. ALX-804-840). Method: Different human normal tissues (A: Stomach; B: Bronchus; C: Vulva/Anal skin; D: Nasopharynx) or cancer tissues (E: Colorectal cancer; F: Malignant glioma) were stained with MAb to lL-33 (Nessy-1) by standard immunohistochemistry. Pictures courtesy of Human Protein Atlas (
http://www.proteinatlas.org/)."
Western Blot analysis of ALX-804-840: Lane 1: MW Marker, Lane 2: IL-33 (human), (recombinant), (Prod. No. ALX-522-098).
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Product Literature References
Endogenous IL-33 Accelerates Metacestode Growth during Late-Stage Alveolar Echinococcosis: B. Autier, et al.; Microbial Spectr.
11, e0423922 (2023),
Abstract;
IL-33/ST2 receptor-dependent signaling in the development of pulmonary hypertension in Sugen/hypoxia mice: C.S. Indralingam, et al.; Physiol. Rep.
1481, e15185 (2022),
Abstract;
NETs decorated with bioactive IL-33 infiltrate inflamed tissues and induce IFN-α production in patients with SL: S. Georgakis, et al.; JCI Insight
6, 147671 (2021),
Abstract;
Anacardic acid induces IL-33 and promotes remyelination in CNS: A. Ljunggren-Rose, et al.; PNAS
117, 21527 (2020),
Abstract;
Immunomodulatory role of Interleukin-33 in large vessel vasculitis: A.C. Desbois, et al.; Sci. Rep.
10, 6405 (2020),
Abstract;
Full Text
Staphylococcus aureus second immunoglobulin-binding protein drives atopic dermatitis via IL-33: A. Al Kindi, et al.; J. Allergy Clin. Immunol.
S0091, 6749 (2020),
Abstract;
TLR3/TAK1 signalling regulates rhinovirus-induced interleukin-33 in bronchial smooth muscle cells: S. Ramu, et al.; ERJ. Open Res.
6, 00147 (2020),
Abstract;
Full Text
Chronic exposure to high fat diet triggers myelin disruption and interleukin-33 upregulation in hypothalamus: H.T Huang, et al.; BMC Neurosci.
20, 33 (2019),
Abstract;
Full Text
Cigarette Smoke Triggers IL-33-Associated Inflammation in a Model of Late Stage COPD: J.H. Lee, et al.; Am. J. Respir. Cell Mol. Biol.
61, 567 (2019),
Abstract;
Full Text
IL-33 drives influenza-induced asthma exacerbations by halting innate and adaptive antiviral immunity: L. Ravanetti, et al.; J. Allergy Clin. Immunol.
143, 1355 (2019),
Abstract;
Interleukin‑33 expression in ovarian cancer and its possible suppression of peritoneal carcinomatosis: A. Sekiya, et al.; Int. J. Oncol.
55, 755 (2019),
Abstract;
Immunopathological Features of Severe Chronic Atopic Keratoconjunctivitis and Effects of Topical Cyclosporine Treatment: C.A. Utine, et al.; Ocul. Immunol. Inflamm.
6, 1 (2018),
Abstract;
Interleukin-33 Predicts Poor Prognosis and Promotes Renal Cell Carcinoma Cell Growth Through its Receptor ST2 and the JNK Signaling Pathway: C.W. Wu, et al.; Cell. Physiol. Biochem.
47, 191 (2018),
Abstract;
Full Text
A novel isoform of IL-33 revealed by screening for transposable element promoted genes in human colorectal cancer: F.E. Lock, et al.; PLoS One
12, e0180659 (2017),
Abstract;
Full Text
IL33 Promotes Colon Cancer Cell Stemness via JNK Activation and Macrophage Recruitment: M. Fang, et al.; Cancer Res.
77, 2735 (2017),
Abstract;
Full Text
Interleukin-33 regulates tissue remodelling and inhibits angiogenesis in the eye: S. Theodoropoulou, et al.; J. Pathol.
241, 45 (2017),
Abstract;
Full Text
Ovatodiolide suppresses allergic airway inflammation and hyperresponsiveness in a murine model of asthma: C.N. Wang, et al.; Eur. J. Pharmacol.
812, 9 (2017),
Abstract;
An antitumorigenic role for the IL-33 receptor, ST2L, in colon cancer: C. O'Donnell, et al. ; Br. J. Cancer
114, 37 (2016),
Abstract;
Full Text
Extracellular IL-33 cytokine, but not endogenous nuclear IL-33, regulates protein expression in endothelial cells: V. Gautier, et al.; Sci. Rep.
6, 34255 (2016),
Application(s): Western blot,
Abstract;
Full Text
Loss of interleukin 33 expression in colonic crypts - a potential marker for disease remission in ulcerative colitis: M.D. Gundersen, et al.; Sci. Rep.
6, 35403 (2016),
Application(s): Immunostaining, paraffin-embedded biopsies ,
Abstract;
Full Text
TLR3 Agonist Poly-IC Induces IL-33 and Promotes Myelin Repair: C. Natarajan, et al.; PLoS One
11, e0152163 (2016),
Application(s): Western blot,
Abstract;
Full Text
Interleukin-33 is released in spinal cord and suppresses experimental autoimmune encephalomyelitis in mice: H. Chen, et al.; Neuroscience
308, 157 (2015),
Application(s): Western Blot,
Abstract;
MicroRNA29a regulates IL-33-mediated tissue remodelling in tendon disease: N. L. Millar, et al.; Nat. Commun.
6, 6774 (2015),
Application(s): Immunohistochemistry,
Abstract;
Full Text
Nuclear IL-33 regulates soluble ST2 receptor and IL-6 expression in primary human arterial endothelial cells and is decreased in idiopathic pulmonary arterial hypertension: D. Shao, et al.; Biochem. Biophys. Res. Commun.
451, 8 (2014),
Application(s): Immunohistochemistry using human paraffin-embedded lung tissue sections; Immunoprecipitation and Western blot using human pulmonary arterial endothelial cells (HPAECs) and lung microvascular endothelial cells (HLMVECs),
Abstract;
The Experimentally Induced Psoriatic Lesion Associates with Rapid but Transient Decrease in Interleukin-33 Immunostaining in Epidermis: M.M. Suttle, et al.; Acta Derm. Venereol.
95, 536 (2014),
Application(s): Immunocytochemistry using human keratinocytes,
Abstract;
Full Text
Unique Temporal and Spatial Expression Patterns of IL-33 in Ovaries during Ovulation and Estrous Cycle Are Associated with Ovarian Tissue Homeostasis: C.I. Carlock, et al.; J. Immunol.
193, 161 (2014),
Application(s): Western blot application,
Abstract;
IL-33 is expressed in epithelia from patients with cystic fibrosis and potentiates neutrophil recruitment: L. Roussel, et al.; J. Allergy Clin. Immunol.
131, 913 (2013),
Application(s): Immunohistochemistry using human lung tissue samples,
Abstract;
Contribution of IL-33-activated type II innate lymphoid cells to pulmonary eosinophilia in intestinal nematode-infected mice: K. Yasuda, et al.; Proc. Natl. Acad. Sci.
109, 3451 (2012),
Application(s): Immunoblotting analysis of the levels of IL-33 protein in the lungs from mice,
Abstract;
Full Text
Interleukin 33 as a Mechanically Responsive Cytokine Secreted by Living Cells: R. Kakkar, et al.; J. Biol. Chem.
287, 6941 (2012),
Abstract;
Full Text
Endogenous IL-33 enhances Th2 cytokine production and T-cell responses during allergic airway inflammation: J. Louten, et al.
; Int. Immunol.
23, 307 (2011),
Application(s): IHC application with murine lung tissue,
Abstract;
Full Text
IL-33 Exacerbates Acute Kidney Injury: A. Akcay, et al.; J. Am. Soc. Nephrol.
22, 2057 (2011),
Application(s): IHC application with Kidney tissues ,
Abstract;
Full Text
Interleukin-33 Induces Expression of Adhesion Molecules and Inflammatory Activation in Human Endothelial Cells and in Human Atherosclerotic Plaques: S. Demyanets, et al.; Arterioscler. Thromb. Vasc. Biol.
31, 2080 (2011),
Abstract;
Full Text
The Dual Function Cytokine IL-33 Interacts with the Transcription Factor NF-kappaB To Dampen NF-kappaB–Stimulated Gene Transcription: S. Ali, et al.; J. Immunol.
187, 1609 (2011),
Abstract;
Full Text
The IL1-like cytokine IL33 and its receptor ST2 are abnormally expressed in the affected skin and visceral organs of patients with systemic sclerosis: M. Manetti, et al.; Ann. Rheum. Dis.
69, 598 (2010),
Abstract;
Caspase-1, Caspase-8, and Calpain Are Dispensable for IL-33 Release by Macrophages: T. Ohno, et al.; J. Immunol.
183, 7890 (2009),
Abstract;
Full Text
IL-33 amplifies the polarization of alternatively activated macrophages that contribute to airway inflammation: M. Kurowska-Stolarska, et al.; J. Immunol.
183, 64649 (2009),
Application(s): Immunohistochemistry application with frozen sections of bronchial tissue
,
Abstract;
Full Text
Increased expression of IL-33 in severe asthma: evidence of expression by airway smooth muscle cells: D. Préfontaine, et al.; J. Immunol.
183, 5094 (2009),
Application(s): Immunocytochemistry application with bronchial tissue samples
,
Abstract;
Full Text
Interleukin-33 Is Biologically Active Independently of Caspase-1 Cleavage: D. Talabot-Ayer, et al.; J. Biol. Chem.
284, 19420 (2009),
Abstract;
Full Text
The IL-1-like cytokine IL-33 is inactivated after maturation by caspase-1: C. Cayrol & J.-P. Girard; PNAS
106, 9021 (2009),
Abstract;
Full Text
Nuclear Interleukin-33 Is Generally Expressed in Resting Endothelium but Rapidly Lost upon Angiogenic or Proinflammatory Activation: A.M. Küchler, et al.; Am. J. Pathol.
173, 1229 (2008),
Abstract;
General Literature References
IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines: J. Schmitz, et al.; Immunity
23, 479 (2005),
Abstract;
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