Product Details
Alternative Name: | NFATc2, Nuclear factor of activated T cells, cytoplasmic 2, NFATP, NFAT1 |
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Clone: | 25A10.D6.D2 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to aa 51-69 (A51ISSPSGLAYPDDVLDYGL69) of mouse nuclear factor of activated T cells, cytoplasmic 2 (NF-ATc2) conserved in all three isoforms A, B and C. |
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UniProt ID: | Q60591 |
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Species reactivity: | Human, Mouse, Rat
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Crossreactivity: | Does not cross-react with NF-ATc1. |
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Applications: | ICC, IF, IHC (FS), IHC (PS), IP, WB Gel Supershift
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Recommended Dilutions/Conditions: | Immunocytochemistry (1:100 - 1:1,000) Immunofluorescence (1:100 - 1:1,000) Immunohistochemistry (frozen sections and paraffin sections, 1:50 - 1:200) Western Blot (1µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects bands of ~140kDa (phosphorylated NF-ATc2 in resting immune cells) and ~120kDa (fully-dephosphorylated NF-ATc2 in stimulated cells) by Western blot. |
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Formulation: | Liquid. Purified antibody in PBS containing 0.05% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Technical Info/Product Notes: | Note: Confusion may arise regarding NFAT protein versus gene names. According to UniProt, the nomenclature is as follows:Nuclear Factor of Activated T Cells, Cytoplasmic 1 (NF-ATc1)Gene Names: NFATC1 or NFATC or NFAT2 Nuclear Factor of Activated T Cells, Cytoplasmic 2 (NF-ATc2)Gene Names: NFATC2 or NFATP or NFAT1 Nuclear Factor of Activated T Cells, Cytoplasmic 3 (NF-ATc3)Gene Names: NFATC3 or NFATX or NFAT4 Nuclear Factor of Activated T Cells, Cytoplasmic 4 (NF-ATc4)Gene Names: NFATC4 or NFAT3 Nuclear Factor of Activated T Cells (NF-AT5)Gene Names: NFAT5 |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
The basis for TCR-mediated regulation of the IL-2 receptor alpha chain gene: role of widely separated regulatory elements: H.P. Kim & W. Leonard; EMBO J.
21, 3051 (2002),
Abstract;
Full Text
Identification and characterization of a novel nuclear factor of activated T-cells-1 isoform expressed in mouse brain: S. Plyte, et al.; J. Biol. Chem.
276, 14350 (2001),
Abstract;
Full Text
The calcineurin-NFAT pathway and muscle fiber-type gene expression: S.J. Swoap, et al.; Am. J. Physiol. Cell Physiol.
279, C915 (2000),
Abstract;
Full Text