Product Details
| Alternative Name: | TNF-R 60kDa, CD120a, p55, TNFRSF 1A |
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| Clone: | H398 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Human TNF-R1. |
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| UniProt ID: | P19438 |
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| Species reactivity: | Human
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| Specificity: | Recognizes membrane-bound and soluble TNF-R1. |
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| Crossreactivity: | Does not cross-react with mouse TNF-R1. |
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| Applications: | Flow Cytometry, IHC (FS), FUNC
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| Recommended Dilutions/Conditions: | Immunohistochemistry (frozen sections; stains acetone-fixed cryostat sections or cell smears. Recommended for H398 is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP)
or peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections. H398 may be used at a concentration of 1-0.5mg/ml for cryostat sections)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Not recommended for IHC (PS).
Functional Application: blocks TNF-R signalling. 10µg/ml H398 neutralizes TNF induced cytotoxicity on U937 (100%). Furthermore, 10µg/ml H398 neutralizes TNF induced MHC expression on K562, Colo 205 and IL2R on YT cells (always 100%).
TNF induced cytotoxicity can be inhibited/neutralized by H398 on MCF-7, HeLa and L929. A working concentration could not be determined, since ascites antibody preparations have been used which exhibit an unspecific cytotoxic effect on the cells. Functional activity via the TNF-R1 is completely inhibited by H398 although on some cells the ligand still binds. Thus, the antibody H398 inhibits signalling via the receptor but not always the binding of TNF. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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