Product Details
| Alternative Name: | Death receptor-6, TNFRSF 21 |
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| Clone: | Luke-1 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant human DR6 (death receptor-6) (aa 42-335). |
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| UniProt ID: | O75509 |
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| Source: | Purified from concentrated hybridoma tissue culture supernatant. |
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| Species reactivity: | Human
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| Applications: | Flow Cytometry
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Apoptosis is induced by certain cytokines including TNF and Fas ligand (CD95L) of the TNF family through their death domain containing receptors, TNF-R1 and Fas (CD95). Several death receptors including DR3, TRAIL-R1, and TRAIL-R2 were identified. A death domain containing receptor in the TNF-R family was cloned and termed DR6 for death receptor-6. Like TNF-R1, DR6 interacts with death domain containing adapter molecule TRADD. Overexpression of DR6 induces apoptosis and activates NF-κB and JNK. DR6 is widely expressed in human tissues and cell lines. The ligand for DR6 has not been identified yet. T cells from DR6-deficient mice showed preference toward Th2 differentiation in vitro, suggesting that DR6, working through JNK functions to attenuate the Th2 response. Other studies of DR6-targeted mutant mice demonstrated that DR6 functions as a regulatory receptor for mediating CD4(+) T cell activation and maintaining proper immune responses. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid coding for the extracellular domain of human DR6 (thick line).Method: Cells (5x105) were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1µg/ml of anti-DR6 MAb (Luke-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.
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General Literature References
Enhanced CD4+ T cell proliferation and Th2 cytokine production in DR6-deficient mice: J. Liu, et al.; Immunity
15, 23 (2001),
Abstract;
Impaired c-Jun amino terminal kinase activity and T cell differentiation in death receptor 6-deficient mice : H. Zhao, et al.; J. Exp. Med.
194, 1441 (2001),
Abstract;
Full Text
Identification and functional characterization of DR6, a novel death domain-containing TNF receptor : G. Pan, et al.; FEBS Lett
431, 351 (1998),
Abstract;
