ODN 1982 (TLRGRADE^®) (synthetic) (Control)

Figure 2: TLR9-dependent mitogenic activity of ODN 1826 (TLRGRADE®) (synthetic) (Prod. No. ALX-746-002) in mouse splenocytes. Method: Culture of 5x10⁵ cells per 200µl well was performed in serum-free conditions after addition of ODNs at the indicated concentrations. Cell proliferation was analyzed after 72 hours with a 16 hours³H-thymidine pulse.Only CpG ODN 1826 (Prod. No. ALX-746-002), but not the corresponding GpC control ODN 1982 (Prod. No. ALX-746-201) induces proliferation of wild type mouse splenocytes. Induction of cell proliferation is absolutely dependent on the presence of TLR9 as addressed with cells derived from TLR9-deficient mice. Note the logarithmic scale for the x and y-axis and the high activity of the active ODNs: max. stimulation is already achieved with 0.010-0.100nmol per 200µl well (10-100nM, corresponding to ~0.5-5ug/ml).