Online Purchasing Account You are logged on as Guest. Login Register a New Account Shopping cart (Empty)

ODN M362 (TLRGRADE^®) (synthetic)

TLR9 ligand



ALX-746-004-C100	100 µg	Inquire for pricing
Do you need bulk/larger quantities?

For a larger pack size, please see Prod. No. ALX-746-054.

CpG oligodeoxynucleotide (Type C) with phosphorothioate backbone. Specific ligand for TLR9 (Toll-like receptor 9).

Product Details

Sequence:	5’-tcgtcgtcgttcgaacgacgttgat-3’(lower case letters indicate phosphorothioate linkage).

MW:	8073 (ammonium salt)

Source:	Synthetic.

Quantity:	12.2nmol (~98µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate).

Formulation:	Lyophilized. Sterile.

Purity Detail:	Activity and endotoxin tested - TLRGRADE^®.

Endotoxin Content:	<0.002EU/µg (LAL test; BioWhittaker)

Reconstitution:	For a 100µM stock solution, dissolve the total vial content in 122µl endotoxin-free water (included) or PBS (to order separately). To obtain optimal dissolving we recommend the following procedure: - Add 50% of the solvent and let dissolve for 10 min. - Add remaining 50% of the solvent and mix thoroughly. - Moderate warming may aid dissolving.

Shipping:	Ambient Temperature

Long Term Storage:	+4°C

Use/Stability:	Aqueous stock solution is stable for 1 day when stored at +4°C.

Handling:	Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.

Technical Info/Product Notes:	Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008). For the inactive control compound, please see ALX-746-204.

Regulatory Status:	RUO - Research Use Only

Product Literature References

Increased regulatory B cells are involved in immune evasion in patients with gastric cancer: Y. Murakami, et al.; Sci. Rep. 9, 13083 (2019), Application(s): PBMCs treatment, Abstract; Full Text

Human blood myeloid and plasmacytoid dendritic cells cross activate each other and synergize in inducing NK cell cytotoxicity: J.J.P. van Beek, et al.; Oncoimmunology 5, e1227902 (2016), Application(s): Human Dendritic Cells stimulation, Abstract; Full Text

Related Products

	iODN (ttaggg)₄ (class II) (endotoxin-free) (synthetic)
	Synthetic. \| Print as PDF

	ALX-746-251-T050	50 tests	128.00 USD
Do you need bulk/larger quantities?

	PBS (endotoxin-free) (sterile)
	Print as PDF

	ALX-505-007-LD15	1.5 ml	67.00 USD

	ALX-505-007-L010	10 ml	132.00 USD
Do you need bulk/larger quantities?

	ddWater (endotoxin-free) (sterile)
	7732-18-5 \| Print as PDF

	ALX-505-008-LD15	1.5 ml	50.00 USD
Do you need bulk/larger quantities?