Replaces Prod. #: ALX-581-014A
Product Details
| Alternative Name: | Lipopolysaccharide from E. coli, Serotype J5 (Rc) |
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| Source: | Rough (R)-form LPS isolated and purified from E. coli J5 (Rc mutant) by a modification of the PCP extraction method, converted to the uniform sodium salt form and dissolved in sterile pyrogen-free double distilled water. |
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| Concentration: | 1mg/ml |
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| Formulation: | Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water. |
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| Purity: | Absence of detectable protein or DNA contaminants with agonistic TLR activity. |
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| Activity: | Strong activator of Toll-like receptor 4 (TLR4). Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice. No further re-extraction required.Smooth (S)-form LPS are commonly the preferred choice for whole animal studies, whereas Rough (R)-form LPS are primarily used in cellular in vitro activation studies. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | +4°C |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at +4°C. |
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| Handling: | Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Use must be restricted to qualified personnel. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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MK2 Inhibitors as a Potential Crohn’s Disease Treatment Approach for Regulating MMP Expression, Cleavage of Checkpoint Molecules and T Cell Activity: E.J. Lebish, et al.; Pharmaceuticals
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Pathogen-selective killing by guanylate-binding proteins as a molecular mechanism leading to inflammasome signaling: S. Feng, et al.; Nat. Commun.
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Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome: D. Fox, et al.; Nat. Commun.
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Full Text
The anti-inflammatory and antioxidant effects of melatonin on LPS-stimulated bovine mammary epithelial cells: G.M. Yu, et al.; PLoS One
12, e0178525 (2017),
Abstract;
Full Text
The Effect of Solar Irradiated Vibrio cholerae on the Secretion of Pro-Inflammatory Cytokines and Chemokines by the JAWS II Dendritic Cell Line In Vitro: C.C. Ssemakalu, et al.; PLoS One
10, e0130190 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
CD14 and TRIF govern distinct responsiveness and responses in mouse microglial TLR4 challenges by structural variants of LPS: T. Regen, et al.; Brain Behav. Immun.
25, 957 (2011),
Abstract;
R-form LPS, the master key to the activation ofTLR4/MD-2-positive cells: M. Huber, et al.; Eur. J. Immunol.
36, 701 (2006),
Abstract;
Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Lüderitz; Methods in Carbohydrate Chemistry
9, 11 (1993),
Abstract;
Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Lüderitz; Eur. J. Biochem.
54, 603 (1975),
Abstract;
A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem.
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