Product Details
| Alternative Name: | CD90 |
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| Source: | Produced in HEK 293 cells. The extracellular domain of human Thy-1 (aa 20-130) is fused to the Fc portion of human IgG1. |
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| UniProt ID: | P04216 |
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| Concentration: | 1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Bio Whittaker). |
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| Reconstitution: | Reconstitute with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | Human Thy-1 is a glycosyl phosphatidylinositol (GPI)- anchored glycoprotein of the immunoglobulin superfamily expressed in various cell types, particularly in cells of the neuronal system, activited microvascular endothelial cells, fibroblasts and blood stem cells. Thy-1 is involved in cell adhesion. Human Thy-1 interacts with the integrin αvβ3 (CD51/CD61) and Mac-1 (CD11b/CD18). In astrocytes, binding of Thy-1 to beta3-integrin promotes focal adhesion formation. Historical results have shown that human Thy-1 binds to human αvβ3 integrin. |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE analysis of Prod. No. ALX-522-091: Lane 1: MW marker, Lane 2: 0.1, Lane 3: 0.5, Labe 4: 1 µg Thy-1 (human):Fc (human), (recombinant).
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Product Literature References
Effect of the GPI anchor of human Thy-1 on antibody recognition and function: J.E. Bradley, et al.; Lab. Invest.
93, 365 (2013),
Abstract;
Full Text
Thy-1-Integrin {alpha}vβ5 Interactions Inhibit Lung Fibroblast Contraction-induced Latent Transforming Growth Factor-β1 Activation and Myofibroblast Differentiation: Y. Zhou, et al.; J. Biol. Chem.
285, 22382 (2010),
Abstract;
Full Text
General Literature References
Interaction of human Thy-1 (CD 90) with the integrin alphavbeta3 (CD51/CD61): an important mechanism mediating melanoma cell adhesion to activated endothelium: A. Saalbach, et al.; Oncogene
24, 4710 (2005),
Abstract;
Human Thy-1 (CD90) on activated endothelial cells is a counterreceptor for the leukocyte integrin Mac-1 (CD11b/CD18): A. Wetzel, et al.; J. Immunol.
172, 3850 (2004),
Abstract;
Thy-1 binds to integrin beta(3) on astrocytes and triggers formation of focal contact sites: L. Leyton, et al.; Curr. Biol.
11, 1028 (2001),
Abstract;
