Product Details
| Alternative Name: | TNFRSF 13C, BR3, BlySR3, CD268 |
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| MW: | ~40kDa (SDS-PAGE) |
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| Source: | Produced in HEK 293 cells. The extracellular domain of human BAFF-R (aa 2-71) is fused at the C-terminus to the Fc portion of human IgG1. |
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| UniProt ID: | Q96RJ3 |
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| Concentration: | 1mg/ml (after reconstitution) |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Bio Whittaker). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds human and mouse BAFF. |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to BAFF.
Detection of membrane-bound human and mouse BAFF in combination with PAb to human IgG1. |
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| Reconstitution: | Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Historical results have shown that BAFF-R inhibits rhsBAFF mediated splenocyte survival. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: rhBAFF-R:Fc inhibits rhsBAFF-mediated activation of freshly isolated mouse splenocytes. Concentration of rhBAFF-R:Fc required to achieve complete inhibition depends on the concentration of rhsBAFF (Prod. No.
ALX-522-025) used. A ratio (rhsBAFF:rhBAFF-R:Fc) 1:50 and higher is recommended.
Method: On day 0 splenocytes were isolated from a freshly collected C57B16 spleen. An aliquot of the splenocytes was analyzed on FACS, and the B-cells were gated on the SSC-FSC panel. FACS settings were saved. The rest of the cells was put in culture with media only, with the indicated concentration of rhsBAFF (Prod. No.
ALX-522-025) alone or in the presence of excess rhsBAFF-R:Fc (+5µg/ml). After 3 days in culture, cells were again analyzed on FACS with the saved settings. Ratio of % living/% dead cells was calculated and plotted."
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Product Literature References
Role of B Cell-Activating Factor in Fibrosis Progression in a Murine Model of Non-Alcoholic Steatohepatitis: K.K. Okada, et al.; Int. J. Mol. Sci.
24, 2509 (2023),
Abstract;
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