Product Details
Alternative Name: | 4-1BB, TNFRSF 9 |
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MW: | ~50kDa (SDS-PAGE). |
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Source: | Produced in HEK 293 cells. The cysteine-rich region of human CD137 (4-1BB) (aa 1-186) is fused to the Fc portion of human IgG1. |
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UniProt ID: | Q07011 |
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Concentration: | 1mg/ml after reconstitution. |
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Formulation: | Lyophilized. Contains PBS. |
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Purity: | ≥95% (SDS-PAGE) |
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Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of Cape Cod). |
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Specificity: | Binds human CD137L (4-1BBL). |
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Applications: | ELISA, FUNC
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Application Notes: | ELISA: binds to CD137L at 10-100 ng/ml. Can be used together with anti-human IgG1 PAb to detect membrane-bound human CD137. Functional Application: Induces proliferation of monocytes. Note: Induction of monocyte proliferation by CD137 required immobilization of the CD137 protein, e.g. by coating of the protein to the surface of the tissue culture dishes. |
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Reconstitution: | Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
CD 137 induces proliferation and endomitosis in monocytes: J. Langstein, et al.; Blood
94, 3161 (1999),
Abstract;
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