Product Details
| Alternative Name: | β-NGF |
| |
| MW: | ~27kDa (dimer). |
| |
| Source: | Produced in E. coli. Dimer of two identical subunits (119 aa) linked together by very strong hydrophobic interactions. |
| |
| CAS: | 86923-98-0 |
| |
| UniProt ID: | P01138 |
| |
| Quantity: | 5µg (determined by amino acid analysis). |
| |
| Formulation: | Lyophilized. No additives. |
| |
| Purity: | ≥98% (HPLC) |
| |
| Biological Activity: | Neurotrophic activity was bioassayed using rat pheochromocytoma PC12 cell cultures over a period of 5-14 days. Activity is in the range of 1-50ng/ml. |
| |
| Reconstitution: | Reconstitue in water or DMEM to a final concentration of at least 10µg/ml and store at -80°C before use. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Use/Stability: | Lyophilized protein is stable for 2-3 weeks when stored at room temperature and for 6-9 months when stored at -20°C.Reconstituted protein is stable for up to 1 week when stored at +4°C and for 4-6 weeks when stored at -80°C. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Scientific Background: | Human β-NGF is responsible for survival, differentiation and maturation of sympathetic and sensory neurons. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Figure: Neurite outgrowth in PC12 cells.
Method: PC12 cells were grown on collagen coated 24-well plates in DMEM medium supplemented with 6% fetal calf serum and 6% horse serum for 24 hours. β-Nerve Growth Factor (human) (rec.) (Prod. No.
ALX-521-005) was addded at the indicated concentrations. The culture media was changed every 2-3 days. The development of neurites over a period of 5 days was visualized by methylene blue staining."
Please mouse over
General Literature References
Neurobiology. Tracking neurotrophin function: A.M. Davies; Nature
368, 193 (1994), (Review),
Abstract;
Neurotrophic factors enter the clinic: M. Barinaga; Science
264, 772 (1994), (Review),
Abstract;
Neurotrophic factors: two are better than one : R. Nishi; Science
265, 1052 (1994), (Review),
Abstract;
Role of neurotrophins in mouse neuronal development: R. Klein; FASEB J.
8, 738 (1994), (Review),
Abstract;
The neurotrophins and their receptors: structure, function, and neuropathology: L.M. Maness, et al.; Neurosci. & Biobehav. Rev.
18, 143 (1994), (Review),
Abstract;
Towards a comprehensive understanding of the trophic support of motoneurons: H. Thoenen, et al.; C.R. Acad. Sci. III
316, 1158 (1993), (Review),
Abstract;
The changing scene of neurotrophic factors : H. Thoenen; TINS
14, 165 (1991), (Review),
Abstract;
