Control compound for rmsMEGACD40L® (Prod. No. ALX-522-120).
Product Details
| Alternative Name: | Tail-Adp, Tail Adiponectin, Adipocyte complement related protein of 30kDa headless |
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| MW: | ~25kDa |
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| Source: | Produced in HEK 293 cells. The collagen domain of mouse ACRP30 (aa 18-111) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |
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| UniProt ID: | Q60994 |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Endotoxin Content: | <0.1EU/µg protein (LAL-test). |
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| Application Notes: | Negative control for fusion proteins containing mouse ACRPheadless. |
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| Reconstitution: | Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored as recommended. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | FLAG is a registered trademark of Sigma-Aldrich Co. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: ACRP30
headless (mouse) does not bind to rhTNF-R1 and rhOX40:Fc in an ELISA assay.
Method: A 96 well ELISA plate was coated O/N at RT with 100 µl of 1 µg/ml rhTNF-R1:Fc (Prod. No.
ALX-522-013) and 1 µg/ml rhOX40:Fc (Prod. No.
ALX-522-022) in PBS. 100 µl of rmsACRP30
headless at 8 µg/ml were mixed with 100 µl 0.5% FCS in PBS in the first well and a series of 1:1 dilutions was prepared for the following wells. Binding of rmsACRP30
headless to the receptor:Fc was detected after 1 hr incubation at 37°C using a goat anti-human HRP conjugated antibody (Jackson lab; 1:1
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