Product Details
| Alternative Name: | OB gene product |
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| MW: | ~16kDa. |
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| Source: | Produced in E. coli. Homologous to human serum leptin. |
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| CAS: | 177404-21-6 |
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| MI: | 14: 5443 |
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| UniProt ID: | P41159 |
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| Formulation: | Lyophilized from 0.0045mM sodium bicarbonate. |
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| Purity: | ≥95% (SDS-PAGE; reducing and non-reducing) |
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| Purity Detail: | The purified recombinant human leptin consists of >93% monomers as determined by gel-filtration chromatography. |
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| Endotoxin Content: | <0.1EU/µg purified protein. |
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| Biological Activity: | Induces proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. |
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| Reconstitution: | Reconstitute with sterile water or 0.4% sodium bicarbonate, pH 8-9. Do not reconstitute to less than 0.1mg/ml. Further dilutions should be made with medium containing 0.1% HSA or BSA. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Reconstituted protein is stable for 4 weeks when stored at +4°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
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| Scientific Background: | Leptin, the product of the ob (obese) gene, is a 16kDa protein consisting of 146 amino acid residues. Leptin is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence reproductive function. |
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| Regulatory Status: | RUO - Research Use Only |
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| Manufacturer: | Manufactured by BioVendor. |
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Product Literature References
Pharmacological inhibition of galectin-1 by lactulose alleviates weight gain in diet-induced obese rats: R. Mukherjee, et al.; Life Sci.
148, 112 (2016),
Application(s): Quantification of plasma,
Abstract;
General Literature References
Leptin and the regulation of body weight in mammals: J.M. Friedman and J.L. Halaas; Nature
395, 763 (1998), review,
Abstract;
Mutation screening and identification of a sequence variation in the human ob gene coding region: R.V. Considine, et al.; BBRC
220, 735 (1996),
Abstract;
Serum immunoreactive leptin concentrations in normal-weight and obese humans : R.V. Considine, et al. ; New Engl. J. Med.
334, 292 (1996),
Abstract;
Effects of the obese gene product on body weight regulation in ob/ob mice : M.A. Pelleymounter, et al. ; Science
269, 540 (1995),
Abstract;
Evidence against either a premature stop codon or the absence of obese gene mRNA in human obesity : R.V. Considine, et al. ; J. Clin. Invest
95, 2986 (1995),
Abstract;
Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects : M. Maffei, et al.; Nature Medicine
1, 1155 (1995),
Abstract;
Overexpression of the obese (ob) gene in adipose tissue of the human subjects : F. Lönnqvist, et al. ; Nature Medicine
1, 950 (1995),
Abstract;
Positional cloning of the mouse obese gene and its human homologue : Y. Zhang, et al.; Nature
372, 425 (1994),
Abstract;
