Product Details
| Alternative Name: | Matrix metalloproteinase 9, Gelatinase B, 92kDa Type IV collagenase |
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| MW: | ~220kDa. |
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| Source: | Isolated from stimulated human neutrophil granulocytes (buffy coat). Disulfide-bridged MMP-9 homodimer. |
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| EC: | 3.4.24.35 |
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| UniProt ID: | P14780 |
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| Formulation: | Liquid. In 50mM Tris-HCl, pH 7.0, containing 200mM sodium chloride, 5mM CaCl2, 1µM ZnCl2, 0.05% BRIJ35, and 0.05% sodium azide. |
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| Purity: | ≥95% (SDS-PAGE, Western blot) |
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| Specific Activity: | ≥1,400mU/mg protein. One unit is defined as the amount of enzyme that hydrolyzes 1µmol Dnp-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. |
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| Scientific Background: | MMP-9 hydrolyzes the extracellular matrix, e.g. collagen types IV, V and IX and gelatin. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Progelatinase B forms from human neutrophils. complex formation of monomer/lipocalin with TIMP-1: H. Kolkenbrock, et al.; Biol. Chem.
377, 529 (1996),
Abstract;
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett.
296, 263 (1992),
Abstract;
Synthetic substrates for vertebrate collagenase: Y. Masui, et al.; Biochem. Med.
17, 215 (1977),
Abstract;
