Product Details
| Alternative Name: | PDIA4, ERP70, protein disulfide isomerase |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 623-638 of mouse ERp72. |
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| UniProt ID: | P08003 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Dog, Guinea pig, Hamster, Monkey, Rabbit
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| Applications: | IF, IHC (PS), WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~72kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Endoplasmic reticulum protein (ERp) 72, a 72 kDa protein localized to the endoplasmic reticulum, is a member of the Protein Disulfide Isomerase (PDI) family of proteins. Together with other ER resident proteins such as BiP, GRP94, and PDI, they serve as the molecular chaperones for proper folding of newly translocated and glycolsylated proteins such as Thyroglobulin (Tg) and Human chorionic gonadotropin (hCG)-beta. |
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| Technical Info/Product Notes: | Recommended by the Human Protein Atlas Organization for IHC (Ensembl No. ENSG00000155660). |
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| Regulatory Status: | RUO - Research Use Only |
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IHC Analysis: Human prostate tissue stained with ERp72, pAb at 10µg/ml.
IHC Analysis: Human placenta tissue stained with ERp72, pAb at 10µg/ml.
IHC Analysis: Human brain, cerebellum tissue stained with ERp72, pAb at 10µg/ml.
Western blot analysis of ERp72: Lane 1: MW Marker, Lane 2: Rat Liver Microsome Extract, Lane 3: Mouse Liver Microsome Extract, Lane 4: Human Liver Microsome Extract.
IHC Analysis: Human testis tissue stained with ERp72, pAb at 10µg/ml.
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Product Literature References
EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD: G. George, et al.; Elife
9, e53455 (2020),
Abstract;
Full Text
Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response: P. Gomez-Fernandez, et al.; Front. Immunol.
9, 2934 (2018),
Abstract;
Full Text
Nitazoxanide inhibits paramyxovirus replication by targeting the Fusion protein folding: role of glycoprotein-specific thiol oxidoreductase ERp57: S. Piacentini, et al.; Sci. Rep.
8, 10425 (2018),
Abstract;
Full Text
Early-onset torsion dystonia: a novel high-throughput yeast genetic screen for factors modifying protein levels of torsinAΔE: L.F. Zacchi, et al.; Dis. Model. Mech.
10, 1129 (2017),
Abstract;
Full Text
Redox sensitivities of global cellular cysteine residues under reductive and oxidative stress: K. Araki, et al.; J. Proteome Res.
15, 2548 (2016),
Application(s): Western blot,
Abstract;
The C-terminal CGHC motif of protein disulfide isomerase supports thrombosis: J. Zhou, et al.; J. Clin. Invest.
125, 4391 (2015),
Application(s): Western blot,
Abstract;
Full Text
∆F508 CFTR interactome remodelling promotes rescue of cystic fibrosis: S. Pankow, et al.; Nature.
528, 510 (2015),
Application(s): Immunofluorescence,
Abstract;
Full Text
Glucose regulation of load-induced mTOR signaling and ER stress in mammalian heart: S. Sen, et al.; J. Am. Heart. Assoc.
2, e004796 (2013),
Application(s): Western blot,
Abstract;
Full Text
Synergistic cooperation of PDI family members in peroxiredoxin 4-driven oxidative protein folding: Y. Sato, et al.; Sci. Rep.
3, 2456 (2013),
Application(s): Western blot,
Abstract;
Full Text
Action of protein disulfide isomerase on proinsulin exit from endoplasmic reticulum of pancreatic β-cells: G. Raipal, et al.; J. Biol. Chem.
287, 43 (2012),
Application(s): Western blot,
Abstract;
Full Text
The VP1 subunit of JC polyomavirus recapitulates early events in viral trafficking and is a novel tool to study polyomavirus entry: C.D. Nelson, et al.; Virology.
428, 30 (2012),
Application(s): Western blot,
Abstract;
Full Text
A possible biochemical link between NADPH oxidase (Nox) 1redox-signalling and ERp72: T. Kamata, et al. ; Biochem. J.
416, 55 (2008),
Application(s): WB, IF using human samples,
Abstract;
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