Product Details
| Alternative Name: | HSTF1, Heat shock factor protein 1 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the N-terminus of human HSF1 phosphorylated at Ser 326. |
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| UniProt ID: | Q00613 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Predicted MW of ~60kDa. Detects a band of 85-95kDa by Western blot depending on hyperphosphorylation of the native protein. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution. |
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| Regulatory Status: | RUO - Research Use Only |
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![[pSer326]HSF1 polyclonal antibody Western blot](https://www.enzolifesciences.com/fileadmin/files/image/SPA-902_WB2.jpg "[pSer326]HSF1 polyclonal antibody Western blot")
Western Blot analysis of Recombinant HSF1 and HSF1 (phospho): Lane 1: MWM, Lane 2: Recombinant HSF1, Lane 3: Recombinant HSF1 (phospho), probed with HSF1 (pSer326) pAb.
![[pSer326]HSF1 polyclonal antibody Western blot](https://www.enzolifesciences.com/fileadmin/files/image/SPA-902_WB1.jpg "[pSer326]HSF1 polyclonal antibody Western blot")
Western blot analysis of HSF1 (pSer326): Lane 1: MW marker, Lane 2: HeLa, Lane 3: HeLa, Heat-Shocked (No recovery time), probed with HSF1 (pSer326) pAb.
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Product Literature References
N-butyrate increases heat shock protein 70 through heat shock factor 1 and AMP-activated protein kinase pathways in human intestinal Caco-2 cells: P.A. Adesina, et al.; Arch. Biochem. Biophys.
736, 109525 (2023),
Abstract;
Propionate and Dietary Fermentable Fibers Upregulate Intestinal Heat Shock protein70 in Intestinal Caco-2 Cells and Mouse Colon: P.A. Adesina, et al.; J. Agric. Food Chem.
69, 8460 (2021),
Abstract;
mTORC2/AKT/HSF1/HuR constitute a feed-forward loop regulating Rictor expression and tumor growth in glioblastoma: B. Holmes, et al.; Oncogene
37, 732 (2018),
Abstract;
Full Text
Branched-chain amino acid catabolism is a conserved regulator of physiological ageing: J. Mansfeld, et al.; Nat. Commun.
6, 10043 (2015),
Application(s): Immunoblotting, supernatants from nematodes,
Abstract;
Full Text
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![[pSer326]HSF1 polyclonal antibody Western blot](https://www.enzolifesciences.com/fileadmin/thumbs/c86a2bcba06e7b7497963ccb7a197764ae64cc61-SPA-902_WB1.jpg.jpg "[pSer326]HSF1 polyclonal antibody Western blot")
