Product Details
| Alternative Name: | CNX |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the sequence near the N-terminus of dog calnexin. |
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| UniProt ID: | P24643 |
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| GenBank ID: | X53616 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, C. elegans, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
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| Applications: | ICC, IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~90kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Calnexin (CNX), an unglycosylated resident ER transmembrane protein, together with Calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans. Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded. Furthermore, Calnexin has been demonstrated to function as a molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins. |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of human placenta tissue stained with Calnexin, pAb at 5µg/ml.
Western blot analysis: Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.
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Product Literature References
N-Glycan on the Non-Consensus N-X-C Glycosylation Site Impacts Activity, Stability, and Localization of the Sda Synthase B4GALNT3: V. Cogez, et al.; Int. J. Mol. Sci.
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Abstract;
PERK recruits E-Syt1 at ER–mitochondria contacts for mitochondrial lipid transport and respiration: M.L. Sassano, et al.; J. Cell Biol.
222, e202206008 (2023),
Abstract;
A role for N-glycosylation in active adenosine deaminase 2 production: M. Ito, et al.; Biochim. Biophys. Acta Gen. Subj.
1866, 130237 (2022),
Abstract;
ATP13A1 prevents ERAD of folding-competent mislocalized and misoriented proteins: M.J. Mkenna, et al.; Mol. Cell
82, 4277 (2022),
Abstract;
Identification and Validation of miR-222-3p and miR-409-3p as Plasma Biomarkers in Gestational Diabetes Mellitus Sharing Validated Target Genes Involved in Metabolic Homeostasis: T. Filardi, et al.; Int. J. Mol. Sci.
23, 4276 (2022),
Abstract;
Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells: T. Kurosaki, et al.; Mol. Cell
82, 4564 (2022),
Abstract;
NF-кB c-Rel modulates pre-fibrotic changes in human fibroblasts: L.C. Micus, et al.; Arch. Dermatol. Res.
314, 943 (2022),
Abstract;
Chronic restraint stress induces changes in the cerebral Galpha 12/13 and Rho-GTPase signaling network: K. Rafa-Zabłocka, et al.; Pharmacol. Rep.
732, 1179 (2021),
Abstract;
Different mRNA expression patterns in keratoglobus and pellucid marginal degeneration keratocytes: T. Stachon, et al.; Exp. Eye Res.
213, 108804 (2021),
Abstract;
Hypoxic stress increases NF-κB and iNOS mRNA expression in normal, but not in keratoconus corneal fibroblasts: T. Stachon, et al.; Graefes Arch. Clin. Exp. Ophthalmol.
259, 449 (2021),
Abstract;
Full Text
Lipid Storage and Interferon Response Determine the Phenotype of Ground Glass Hepatocytes in Mice and Humans: Y. Churin, et al.; Cell. Mol. Gastroenterol. Hepatol.
12, 383 (2021),
Abstract;
EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD: G. George, et al.; Elife
9, e53455 (2020),
Abstract;
Full Text
Soluble dimeric prion protein ligand activates Adgrg6 receptor but does not rescue early signs of demyelination in PrP-deficient mice: A. Henzi, et al.; PLoS One
15, e0242137 (2020),
Abstract;
Full Text
Fam20C-mediated phosphorylation of osteopontin is critical for its secretion but dispensable for its action as a cytokine in the activation of hepatic stellate cells in liver fibrogenesis: E. Tibaldi, et al.; FASEB J.
34, 1122 (2019),
Abstract;
Full Text
Identification of calnexin as a diacylglycerol acyltransferase-2 interacting protein: C. Brandt, et al.; PLoS One
14, e0210396 (2019),
Abstract;
Full Text
Misfolded GPI-anchored proteins are escorted through the secretory pathway by ER-derived factors: E. Zavodszky & R.S. Hegde; Elife
8, e46740 (2019),
Abstract;
Full Text
Control of insulin granule formation and function by the ABC transporters ABCG1 and ABCA1 and by oxysterol binding protein OSBP: S.S. Hussain, et al.; Mol. Biol. Cell
29, 1238 (2018),
Application(s): Isoosmotic cell fractionation,
Abstract;
Full Text
EMC Is Required to Initiate Accurate Membrane Protein Topogenesis: P.J. Chitwood, et al.; Cell
175, 1507 (2018),
Abstract;
Full Text
LST-3TM12 is a member of the OATP1B family and a functional transporter: V. Malagnino, et al.; Biochem. Pharmacol.
148, 75 (2018),
Abstract;
Mitochondrial CDP-diacylglycerol synthase activity is due to the peripheral protein, TAMM41 and not due to the integral membrane protein, CDP-diacylglycerol synthase 1: N.J. Blunsom, et al.; Biochim. Biophys. Acta Mol. Cell Biol. Lipids
1863, 284 (2018),
Abstract;
Full Text
The binding of TBK1 to STING requires exocytic membrane traffic from the ER: E. Ogawa, et al.; Biochem. Biophys. Res. Commun.
503, 138 (2018),
Abstract;
The scaffold protein PDZK1 modulates expression and function of the organic anion transporting polypeptide 2B1: C. Ferreira, et al.; Eur. J. Pharm. Sci.
120, 181 (2018),
Abstract;
CLN5 is cleaved by members of the SPP/SPPL family to produce a mature soluble protein: F. Jules, et al.; Exp. Cell. Res.
357, 40 (2017),
Abstract;
Fenofibrate, but not ezetimibe, prevents fatty liver disease in mice lacking phosphatidylethanolamine N-methyltransferase: J.N. van der Veen, et al.; J. Lipid. Res.
58, 656 (2017),
Application(s): Western blot,
Abstract;
Full Text
Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission: P. Leblanc, et al.; Methods Mol. Biol.
1545, 153 (2017),
Abstract;
Cannabinoid receptor subtype 2 (CB2R) agonist, GW405833 reduces agonist-induced Ca2+ oscillations in mouse pancreatic acinar cells: Z. Huang, et al.; Sci. Rep.
6, 29757 (2016),
Application(s): CB2R Immunoblot Assay,
Abstract;
Full Text
Epigenetic Regulation of Steroid Inactivating UDP-Glucuronosyltransferases by microRNAs in Prostate Cancer: G. Margaillan, et al.; J. Steroid Biochem. Mol. Biol.
155, 85 (2016),
Application(s): Protein immunodetection,
Abstract;
Human Cytomegalovirus gH/gL Forms a Stable Complex with the Fusion Protein gB in Virions: A.L. Vanarsdall, et al.; PLoS Pathog.
12, e1005564 (2016),
Application(s): Western Blot,
Abstract;
Full Text
Mannosidase IA is in Quality Control Vesicles and Participates in Glycoprotein Targeting to ERAD: N. Ogen-Shtern, et al.; J. Mol. Biol.
428, 3194 (2016),
Application(s): Immunoblotting,
Abstract;
Probing functional changes in exocyst configuration with monoclonal antibodies: S.M. Inamdar, et al.; Front. Cell. Dev. Biol.
4, 51 (2016),
Application(s): Immunofluorescence,
Abstract;
Full Text
Sphingosine-1-Phosphate Lyase Deficient Cells as a Tool to Study Protein Lipid Interactions: M.J. Gerl, et al.; PLoS One
11, e0153009 (2016),
Application(s): Loading control,
Abstract;
Full Text
A Japanese encephalitis virus genotype 5 molecular clone is highly neuropathogenic in a mouse model: impact of the structural protein region on virulence: M. de Wispelaere, et al.; J. Virol.
89, 5862 (2015),
Application(s): Western blot,
Abstract;
Full Text
A single amino acid substitution in the M protein attenuates Japanese Encephalitis virus in mammalian hosts: M. de Wispelaere, et al.; J. Virol.
90, 2676 (2015),
Application(s): Western blot,
Abstract;
Full Text
A STIM2 splice variant negatively regulates store-operated calcium entry: A. M. Miederer, et al.; Nat. Commun.
6, 6899 (2015),
Abstract;
Full Text
Development-dependent regulation of molecular chaperones after hypoxia-ischemia: X. Sun, et al.; Neurobiol. Dis.
82, 123 (2015),
Abstract;
Disrupted-in-schizophrenia-1 (DISC1) Regulates Endoplasmic Reticulum Calcium Dynamics: S.J. Park, et al.; Sci. Rep.
5, 8694 (2015),
Application(s): Immunogold Electron Microscopy,
Abstract;
Full Text
Exosomes, endogenous retroviruses and toll-like receptors: pregnancy recognition in ewes: I. Ruiz-Gonzalez, et al.; Reproduction
149, 281 (2015),
Application(s): Western blot,
Abstract;
Full Text
Insufficient glucose supply is linked to hypothermia upon cold exposure in mice lacking phosphatidylethanolamine N-methyltransferase: X. Gao, et al.; J. Lipid. Res.
56, 1701 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Neuroprotective effects of transcription factor Brn3b in an ocular hypertension rat model of glaucoma: D.L. Stankowska, et al.; Invest. Ophthalmol. Vis. Sci.
56, 893 (2015),
Application(s): Western Blotting,
Abstract;
Full Text
Reduced Fertility and Altered Epididymal and Sperm Integrity in Mice Lacking ADAM7: H. Choi, et al.; Biol. Reprod.
93, 70 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Role for Rab10 in Methamphetamine-Induced Behavior: S.M. Vanderwerf, et al.; PLoS One
10, e0136167 (2015),
Application(s): Western blot,
Abstract;
Full Text
Targeting membrane proteins to inner segments of vertebrate photoreceptors: Y. Pan; (2015), (PhD thesis),
Application(s): Immunostaining,
Abstract;
Full Text
UBC9-dependent association between calnexin and protein tyrosine phosphatase 1B (PTP1B) at the endoplasmic reticulum: D. Lee, et al.; J. Biol. Chem.
290, 5725 (2015),
Abstract;
Full Text
Transcriptional regulation of apolipoprotein A-IV by the transcription factor CREBH: X. Xu, et al.; J. Lipid. Res.
55, 850 (2014),
Application(s): Western blot,
Abstract;
Full Text
Morphological and biochemical characterization of the membranous hepatitis C virus replication compartment: D. Paul, et al.; J. Virol.
87, 10612 (2013),
Application(s): Immunofluorescence and Western blot,
Abstract;
Full Text
Catalytic properties of MGAT3, a putative triacylgycerol synthase: Y. Shi, et al. ; J. Lipid Res.
48, 583 (2007),
Application(s): ICC using monkey tissue culture,
Abstract;
Caenorhabditis elegans reticulon interacts with RME-1 during embryogenesis: T. Toyoda, et al. ; Biochem. Biophys. Res. Commun.
293, 698 (2002),
Application(s): WB using C. elegans samples,
Abstract;
Luman, the cellular counterpart of herpes simplex virus VP16, is processed by regulated intramembrane proteolysis: V. Misra, et al. ; Mol. Cell. Biol.
22, 5639 (2002),
Application(s): WB using monkey samples,
Abstract;
The glycoprotein gp48 of murine cytomegalovirus: proteasome-dependent cytosolic dislocation and degradation: U.H. Koszinowski, et al. ; J. Biol. Chem.
277, 2216 (2002),
Application(s): ICC using mouse samples,
Abstract;
Herp, a new ubiquitin-like membrane protein induced by endoplasmic reticulum stress: T. Miyata, et al. ; J. Biol. Chem.
275, 32846 (2000),
Application(s): WB using human samples,
Abstract;
Protein-disulfide isomerase (PDI) in FRTL5 cells - pH-dependent thyroglobulin/PDI interactions determine a novel PDI function in the post-endoplasmic reticulum of thyrocytes: R. Miquelis, et al. ; J. Biol. Chem.
275, 1920 (2000),
Application(s): ICC, WB using rat samples,
Abstract;
Calnexin and the immunoglobulin binding protein (BiP) coimmunoprecipitate with AMPA receptors: R.J. Wenthold, et al. ; J. Neurochem.
73, 942 (1999),
Application(s): IP using rat samples,
Abstract;
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