Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~90kDa by Western blot.
Purity Detail:
Protein G affinity purified.
Formulation:
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Long Term Storage:
-20°C
Scientific Background:
The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.
Regulatory Status:
RUO - Research Use Only
Immunohistochemistry analysis of human spleen tissue stained with HSP90, mAb (16F1) at 10µg/ml.
Western blot analysis of HSP90: Lane 1: MW marker, Lane 2: HSP90 native protein (ADI-SPP-770), Lane 3: HSP90b recombinant protein (Prod. No. ADI-SPP-772), Lane 4: HSP90alpha recombinant protein (Prod. No. ADI-SPP-776), Lane 5: HeLa (HS), Lane 6: L-929 (HS), Lane 7: Rat-2 (HS), Lane 8:RK-13 (HS).
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Product Literature References
A novel C-terminal heat shock protein 90 inhibitor that overcomes STAT3-Wnt-β-catenin signaling-mediated drug resistance and adverse effects: H.J. Lee, et al.; Theranostics 12, 105 (2022), Abstract;
A specialized Hsp90 co-chaperone network regulates steroid hormone receptor response to ligand: S.J. Backe, et al.; Cell Rep. 40, 111039 (2022), Abstract;
Improvement in heat stress-induced multiple organ dysfunction and intestinal damage through protection of intestinal goblet cells from prostaglandin E1 analogue misoprostol: H.P. Hii, et al.; Life Sci. 310, 121039 (2022), Abstract;
Tankyrase-mediated ADP-ribosylation is a regulator of TNF-induced death: L. Liu, et al.; Sci. Adv. 8, eabh2332 (2022), Abstract;
Therapeutic Effect of Acer tegmentosum Maxim Twig Extract in Bile Duct Ligation-Induced Acute Cholestasis in Mice: U.J. Bae, et al.; J. Med. Food 25, 652 (2022), Abstract;
Assessing BRCA1 activity in DNA damage repair using human induced pluripotent stem cells as an approach to assist classification of BRCA1 variants of uncertain significance: M. Ozgencil, et al.; PLoS One 16, e0260852 (2021), Abstract;
Hsp90 chaperone code and the tumor suppressor VHL cooperatively regulate the mitotic checkpoint: M.R. Woodford, et al.; Cell Stress Chaperones 26, 965 (2021), Abstract;
Identification of Niemann-Pick C1 protein as a potential novel SARS-CoV-2 intracellular target: I. García-Dorival, et al.; Antiviral Res. 194, 105167 (2021), Abstract;
Hypoxia induced hERG trafficking defect linked to cell cycle arrest in SH-SY5Y cells: D.R. Vaddi, et al.; PLoS One 14, e0215905 (2019), Abstract; Full Text
Post-translational Regulation of FNIP1 Creates a Rheostat for the Molecular Chaperone Hsp90: R.A. Sager, et al.; Cell Rep. 26, 1344 (2019), Abstract; Full Text
Detection and Analysis of Extracellular Hsp90 (eHsp90): S. Cortes, et al.; Methods Mol. Biol. 1709, 321 (2018), Abstract;
Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients: M. Wattin, et al.; Int. J. Mol. Sci. 19, E178 (2018), Application(s):Western Blot, Abstract; Full Text
Selection of stable expressed reference genes in native and vitrified/thawed human ovarian tissue for analysis by qRT-PCR and Western blot: D.A. Nikishin, et al.; J. Assist. Reprod. Genet. 35, 1851 (2018), Abstract;
Sporadic renal angiomyolipoma in a patient with Birt-Hogg-Dubé: chaperones in pathogenesis: R.A. Sager, et al.; Oncotarget 9, 22220 (2018), Abstract; Full Text
Histone deacetylase activity mediates acquired resistance towards structurally diverse HSP90 inhibitors: R.C. Chai, et al.; Mol. Oncol. 11, 567 (2017), Abstract; Full Text
HSP90 activity is required for MLKL oligomerisation and membrane translocation and the induction of necroptotic cell death: A.C. Jacobsen, et al.; Cell Death Dis. 14, e2051 (2016), Application(s): Western blots of both mouse and human cell lines, Abstract;
HSP90 regulates temperature-dependent seedling growth in Arabidopsis by stabilizing the auxin co-receptor F-box protein TIR1: R. Wang, et al.; Nat. Commun. 7, 10269 (2016), Application(s): Immunoprecipitation, Abstract;
Inverse Relationship between Progesterone Receptor and Myc in Endometrial Cancer: T. Kavlashvili, et al.; PLoS One 11, e0148912 (2016), Abstract; Full Text
Recombinant HSP70 and mild heat shock stimulate growth of aged mesenchymal stem cells: N.V. Andreeva, et al.; Cell Stress Chaperones 21, 727 (2016), Abstract; Full Text
Human Genetic Relevance and Potent Antitumor Activity of Heat Shock Protein 90 Inhibition in Canine Lung Adenocarcinoma Cell Lines: F. Clemente-Vicario, et al.; PLoS One 10, e0142007 (2015), Application(s): Immunoblot analyses, Abstract; Full Text
Identification and quantitative analysis of cellular proteins affected by treatment with withaferin A using A SILAC-based proteomics approach: M. Narayan, et al.; J. Ethnopharmacol. 8741, 30149 (2015), Application(s): Western Blot, Abstract;
Prion-like transmission of neuronal huntingtin aggregates to phagocytic glia in the Drosophila brain: M. M. Pearce, et al.; Nat. Commun. 6, 6768 (2015), Application(s): Immunofluorescence , Abstract;
The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling: L. Wu, et al.; J. Immunol. 193, 1590 (2014), Abstract;
Psoriasis-associated variant Act1 D10N with impaired regulation by Hsp90: C. Wang, et al.; Nat. Immunol. 14, 72 (2013), Application(s): WB using MEF and HEK293 extracts, Abstract; Full Text
Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase: M.G. Hasham, et al.; J. Immunol. 189, 2374 (2012), Application(s): WB using mouse splenic B-cells extracts, Abstract;
Methylglyoxal alters the function and stability of critical components of the protein quality control: C.F. Bento, et al.; PLoS One 5, e13007 (2010), Application(s): WB using human cell lysates, Abstract; Full Text
Amyloid-β 1-42 induced endocytosis and clusterin/apoJ protein accumulation in cultured human astrocytes: A. Salminen, et al. ; Neurochem. Int. 50, 540 (2007), Application(s): WB using human cell lysates, Abstract;
Developmental changes of heat-shock proteins in porcine testis by a proteomic analysis: S.Y. Huang, et al.; Theriogenology 64, 1940 (2005), Application(s): Immunohistochemistry on testis tissue, Abstract;
