Product Details
| Alternative Name: | Heat shock protein 70, Hsc70, Hsp73 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to a portion of human Hsc70 (Hsp73). The sequence is completely conserved in rat, mouse, hamster and bovine. |
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| UniProt ID: | P11142 |
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| GenBank ID: | Y00371 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep
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| Applications: | IHC (PS), WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~73kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway. |
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| Regulatory Status: | RUO - Research Use Only |
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Western Blot Analysis of Hsc70 (Hsp73): Lane 1: MWM, Lane 2: HSC70/HSP70 (bovine), (recombinant) (Cat #: ADI-SPP-751) Lane 3: HSP70/HSP72 (human), (recombinant) (Cat #: ADI-NSP-555), Lane 4: HeLa Cell Lysate, Lane 5: L929 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: CHO-K1 Cell Lysate, Lane 8: RK-13 Cell Lysate
Immunohistochemistry analysis of human spleen tissue stained with HSC70/HSP73, pAb at 10µg/ml.
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Product Literature References
Caspase Inhibition Modulates Monocyte-Derived Macrophage Polarization in Damaged Tissues: S. Solier, et al.; Int. J. Mol. Sci.
24, 4151 (2023),
Abstract;
A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating: Y.X. Ni, et al.; Cell Discov.
4, 65 (2018),
Abstract;
Full Text
Heat shock promotes inclusion body formation of mutant huntingtin (mHtt) and alleviates mHtt-induced transcription factor dysfunction: J.Y. Chen, et al.; Jl Biol. Chem.
293, 15581 (2018),
Abstract;
Full Text
Model Based Targeting of IL-6-Induced Inflammatory Responses in Cultured Primary Hepatocytes to Improve Application of the JAK Inhibitor Ruxolitinib: S. Sobotta, et al.; Front. Physiol.
8, 775 (2017),
Abstract;
HSPB8 and the Co-Chaperone BAG3 Are Highly Expressed During the Synthetic Phase of Rat Myometrium Programming During Pregnancy: N.M. Marsh, et al.; Biol. Reprod.
92, 131 (2015),
Application(s): Western Blot,
Abstract;
Full Text
AICAR-induced activation of AMPK negatively regulates myotube hypertrophy through the HSP72-mediated pathway in C2C12 skeletal muscle cells: T. Egawa, et al.; Am. J. Physiol. Endocrinol. Metab.
306, E344 (2014),
Application(s): WB using mouse cell lysate,
Abstract;
Full Text
Rictor/mTORC2 facilitates central regulation of energy and glucose homeostasis: H.E. Kocalis, et al.; Mol. Metab.
3, 394 (2014),
Application(s): Western-blot using mouse tissue,
Abstract;
Full Text
Rescue of atypical protein kinase C in epithelia by the cytoskeleton and Hsp70 family chaperones: P. Salas, et al. ; J. Cell. Sci.
122, 2491 (2009),
Application(s): WB using human & mouse cell lysates & tissue,
Abstract;
Downregulation of renal TonEBP in hypokalemic rats: U.S. Jeon, et al.; Am. J. Physiol. Renal Physiol.
293, F408 (2007),
Application(s): WB using rat tissue,
Abstract;
Tid1 Isoforms Are Mitochondrial DnaJ-like Chaperones with Unique Carboxyl Termini That Determine Cytosolic Fate: B. Lu, et al. ; J. Biol. Chem.
281, 13150 (2006),
Application(s): WB using rabbit samples,
Abstract;
Priming biologically active antibody responses against an isolated, conformational viral epitope by DNA vaccination: R. Schirmbeck, et al. ; J. Immunol.
169, 1251 (2002),
Application(s): WB using human samples,
Abstract;
Cancer-associated retinopathy induced by both anti-recoverin and anti-hsc70 antibodies in vivo: I. Maruyama, et al. ; Invest. Ophthalmol. Vis. Sci.
40, 3160 (1999),
Application(s): IHC, WB using rat samples,
Abstract;
