Product Details
| Alternative Name: | Heat shock protein 70, Hsc70, Hsp73 |
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| Clone: | 1B5 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Native hamster Hsc70 (Hsp73). |
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| UniProt ID: | P19378 |
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| GenBank ID: | M34561 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Turtle
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| Applications: | ELISA, Flow Cytometry, ICC, IF, IHC (PS), IP, WB
Electron microscopy
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~73kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway. |
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| Technical Info/Product Notes: | Cited samples:
For an overview on cited samples please click here. |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of human spleen tissue stained with HSC70/HSP73, mAb (1B5) at 10µg/ml.
Western blot analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) (Prod No. ADI-SPP-751) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) (Prod No. ADI-LYC-3T100) Lane 5: PC-12, (Cell Lysate), (Prod No. ADI-LYC-PC100), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate) (Prod No. ADI-LYC-HL100).
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Product Literature References
Extracellular vesicles from triple negative breast cancer promote pro-inflammatory macrophages associated with better clinical outcome: M. Tkach, et al.; PNAS
119, e2107394119 (2022),
Abstract;
FEZ1 phosphorylation regulates HSPA8 localization and interferon-stimulated gene expression: V. Malikov, et al.; Cell Rep.
38, 110396 (2022),
Abstract;
HSC70 is a novel binding partner involved in the capture of immunoglobulins on B cells in the NOD mouse: E. Renman, et al.; Autoimmunity
55, 520 (2022),
Abstract;
Impaired Heat Shock Protein Expression in Activated T Cells in B-Cell Lymphoma: Z. Albakova, et al.; Biomedicines
10, 2747 (2022),
Abstract;
Lack of Hikeshi activates HSF1 activity under normal conditions and disturbs the heat-shock response: S. Kose, et al.; Life Sci. Alliance
5, e202101241 (2022),
Abstract;
The histone deacetylase inhibitor M344 as a multifaceted therapy for pancreatic cancer: S.M. Knoche, et al.; PLoS One
17, e0273518 (2022),
Abstract;
Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle: Y. Wakabayashi, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
318, R677 (2020),
Abstract;
Endogenous retrovirus gag antigen and its gene variants are unique autoantigens expressed in the pancreatic islets of non-obese diabetic mice: Y.D. Dai, et al.; Immunol. Lett.
223, 62 (2020),
Abstract;
Enniatin B1-induced lysosomal membrane permeabilization in mouse embryonic fibroblasts: C.A.F. Oliveira, et al.; Mycotoxin Res.
36, 23 (2020),
Abstract;
Exosomes influence the behavior of human mesenchymal stem cells on titanium surfaces: X. Wang, et al.; Biomaterials
230, 119571 (2020),
Application(s): WB,
Abstract;
Heat shock factor 2 protects against proteotoxicity by maintaining cell-cell adhesion: J. Joutsen, et al.; Cell Rep.
30, 583 (2020),
Application(s): Western-blot using U2OS cells and mouse embryonic fibroblasts,
Abstract;
Vesicle clustering in a living synapse depends on a synapsin region that mediates phase separation: A. Pechstein, et al.; Cell Rep.
30, 2594 (2020),
Abstract;
Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis: J.V. Ferreira, et al.; PLoS One
15, e0223790 (2019),
Abstract;
Full Text
HDAC4 controls muscle homeostasis through deacetylation of myosin heavy chain, PGC-1α, and Hsc70: L. Luo, et al.; Cell Rep.
29, 749 (2019),
Application(s): Immunoprecipitation using C2C12 cells,
Abstract;
High-throughput screening discovers antifibrotic properties of haloperidol by hindering myofibroblast activation: M. Rehman, et al.; JCI Insight.
4, 123987 (2019),
Abstract;
Full Text
Sphingosine kinase 1 overexpression induces MFN2 fragmentation and alters mitochondrial matrix Ca2+ handling in HeLa cells: I. Pulli, et al.; Biochim. Biophys. Acta Mol. Cell. Res.
1866, 1475 (2019),
Application(s): Western-blot using HeLa and FTC-133 cells,
Abstract;
Tau protein aggregates inhibit the protein-folding and vesicular trafficking arms of the cellular proteostasis network: A. Yu, et al.; J. Biol. Chem.
294, 7917 (2019),
Abstract;
Alcohol exposure promotes DNA methyltransferase DNMT3A upregulation through reactive oxygen species-dependent mechanisms: F. Miozzo, et al.; Cell Stress Chaperones
23, 115 (2018),
Abstract;
Full Text
Disrupted structure and aberrant function of CHIP mediates the loss of motor and cognitive function in preclinical models of SCAR16: C.H. Shi, et al.; PLoS Genet.
14, e1007664 (2018),
Abstract;
Full Text
Functional genomics study of acute heat stress response in the small yellow follicles of layer-type chickens: C.Y. Cheng, et al.; Sci. Rep.
8, 1320 (2018),
Abstract;
Full Text
HSC70 is a chaperone for wild-type and mutant cardiac myosin binding protein C: A.A. Glazier, et al.; JCI Insight
3, 99319 (2018),
Abstract;
Full Text
Identification of E6/E7-Dependent MicroRNAs in HPV-Positive Cancer Cells: A. Honegger, et al.; Methods Mol. Biol.
1699, 119 (2018),
Abstract;
Melanoma LAMP-2C Modulates Tumor Growth and Autophagy: L. Perez, et al.; Front Cell Dev Biol
6, 101 (2018),
Abstract;
Full Text
Modulation of Alzheimer's amyloid β peptide oligomerization and toxicity by extracellular Hsp70: I. Rivera, et al.; Cell Stress Chaperones
23, 269 (2018),
Abstract;
Full Text
Zinc Deficiency via a Splice Switch in Zinc Importer ZIP2/SLC39A2 Causes Cystic Fibrosis-Associated MUC5AC Hypersecretion in Airway Epithelial Cells: S. Kamei, et al.; EBioMedicine
27, 304 (2018),
Abstract;
Full Text
In vitro characterization of six STUB1 variants in spinocerebellar ataxia 16 reveals altered structural properties for the encoded CHIP proteins: Y. Pakdaman, et al.; Biosci. Rep.
32, BSR20170251 (2017),
Abstract;
Full Text
Long-term treatment with EGFR inhibitor erlotinib attenuates renal inflammatory cytokines but not nephropathy in Alport syndrome mouse model: K. Omachi, et al.; Clin. Exp. Nephrol.
21, 952 (2017),
Abstract;
ARD1-mediated Hsp70 acetylation balances stress-induced protein refolding and degradation: J.H. Seo, et al.; Nat. Commun.
7, 12882 (2016),
Application(s): In vitro acetylation assay, deacetylation assay, binding assay, and ATPase activity assay,
Abstract;
Full Text
Astrocytes Surviving Severe Stress Can Still Protect Neighboring Neurons from Proteotoxic Injury: A.M. Gleixner, et al.; Mol. Neurobiol.
53, 4939 (2016),
Abstract;
Full Text
Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development: K. Uchiyama, et al.; Sci. Rep.
6, 32094 (2016),
Application(s): Western blot, mouse colonic epithelial YAMC cells,
Abstract;
Full Text
Engineering hybrid exosomes by membrane fusion with liposomes: Y.T. Sato, et al.; Sci. Rep.
6, 21933 (2016),
Application(s): Western blot,
Abstract;
Full Text
Loss of Panx1 Impairs Mammary Gland Development at Lactation: Implications for Breast Tumorigenesis: M.K. Stewart, et al.; PLoS One
11, e0154162 (2016),
Application(s): Western blot,
Abstract;
Full Text
Recombinant HSP70 and mild heat shock stimulate growth of aged mesenchymal stem cells: N.V. Andreeva, et al.; Cell Stress Chaperones
21, 727 (2016),
Abstract;
Full Text
Systematic Proteomic Identification of the Heat Shock Proteins (Hsp) that Interact with Estrogen Receptor Alpha (ERα) and Biochemical Characterization of the ERα-Hsp70 Interaction: A.E. Dhamad, et al.; PLoS One
11, e0160312 (2016),
Abstract;
Full Text
β-Secretase 1's Targeting Reduces Hyperphosphorilated Tau, Implying Autophagy Actors in 3xTg-AD Mice: D. Piedrahita, et al.; Front Cell Neurosci.
9, 498 (2016),
Application(s): Western blot,
Abstract;
Full Text
Dependence of Intracellular and Exosomal microRNAs on Viral E6/E7 Oncogene Expression in HPV-positive Tumor Cells: A. Honegger, et al.; PLoS Pathog.
11, e1004712 (2015),
Application(s): Western Blotting,
Abstract;
Full Text
K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy: J. Vasco Ferreira, et al.; Sci. Rep.
5, 10210 (2015),
Application(s): Immunoprecipitation,
Abstract;
Full Text
Keratin 8 absence downregulates colonocyte HMGCS2 and modulates colonic ketogenesis and energy metabolism: T.O. Helenius, et al.; Mol. Biol. Cell
26, 2298 (2015),
Application(s): Western Blot,
Abstract;
Full Text
NDR Kinases Are Essential for Somitogenesis and Cardiac Looping during Mouse Embryonic Development: D. Schmitz-Rohmer, et al.; PLoS One
10, e0136566 (2015),
Application(s): Western Blot,
Abstract;
Full Text
HSF1 deficiency and impaired HSP90-dependent protein folding are hallmarks of aneuploid human cells: N. Donnelly, et al.; EMBO J.
33, 2374 (2014),
Abstract;
Full Text
Impact of aging on heat shock protein expression in the substantia nigra and striatum of the female rat.: A.M. Gleixner, et al.; Cell Tissue Res.
357, 43 (2014),
Application(s): Western blot using rat substantia nigra and striatium tissue samples,
Abstract;
STUB1 mutations in autosomal recessive ataxias–evidence for mutation-specific clinical heterogeneity: K. Heimdal, et al.; Orphanet. J. Rare Dis.
9, 146 (2014),
Application(s): Western blot using in vitro ubiquitination assays,
Abstract;
Full Text
Silencing of human papillomavirus (HPV) E6/E7 oncogene expression affects both the contents and the amounts of extracellular microvesicles released from HPV-positive cancer cells: A. Honegger, et al.; Int. J. Cancer
133, 1631 (2013),
Application(s): Western blot using human HeLa cervical carcinoma cells,
Abstract;
Full Text
Basal and stress-induced Hsp70 are modulated by ataxin-3: C.P. Reina, et al.; Cell Stress Chaperones
17, 729 (2012),
Application(s): WB using mouse cell lysate,
Abstract;
Full Text
Methylglyoxal alters the function and stability of critical components of the protein quality control: C.F. Bento, et al.; PLoS One
5, e13007 (2010),
Application(s): WB using human cell lysates,
Abstract;
Full Text
Association of Simian Virus 40 Vp1 with 70-Kilodalton Heat Shock Proteins and Viral Tumor Antigens: H. Kasamatsu, et al. ; J. Virol.
83, 37 (2008),
Application(s): WB, IP, IHC using monkey cell lysates,
Abstract;
Exogenous androgen treatment delays the stress response following hamster facial nerve injury: K. Jones, et al. ; J. Neuroendocrinol.
19, 383 (2007),
Application(s): IHC using hamster tissue,
Abstract;
Fever-like hyperthermia controls T lymphocyte persistence by inducing degradation of cellular FLIPshort: J. Eriksson, et al. ; J. Immunol.
178, 3944 (2007),
Application(s): WB using human cell lysates,
Abstract;
Heat shock protein 70 interacts with aquaporin-2 and regulates its trafficking: D. Brown, et al. ; J. Biol. Chem.
282, 28721 (2007),
Application(s): WB, IP, IF, EM, ICC using rat cell lysates & tissue,
Abstract;
Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases: S. Chen & I.R. Brown; Cell Stress Chaperones
12, 51 (2007),
Application(s): WB, IF using rat tissue,
Abstract;
Heat-shock protein90 complexes in resting and thrombin-activated platelets: R.L. Gear, et al. ; Biochem. Biophys. Res. Commun.
297, 129 (2002),
Application(s): IP using human samples,
Abstract;
Molecular chaperones enhance the degradation of expanded polyglutamine repeat androgen receptor in a cellular model of spinal and bulbar muscular atrophy: D.E. Merry, et al. ; Hum. Mol. Genet.
11, 515 (2002),
Application(s): ICC using mouse samples,
Abstract;
Two distinct pathways mediated by PA28 and hsp90 in major histocompatibility complex class I antigen processing: H. Udono, et al. ; J. Exp. Med.
196, 185 (2002),
Application(s): WB using mouse samples,
Abstract;
The synthetic peptide P-197 inhibits human T-cell leukemia virus type 1 envelope-mediated syncytium formation by a mechanism that is independent of Hsc70: S.R. Jassal, et al. ; J. Virol.
75, 10472 (2001),
Application(s): Flow using human samples,
Abstract;
HSC70 interactions with SV40 viral proteins differ between permissive and nonpermissive mammalian cells: I. Lazaridis, et al. ; Cell Stress Chaperones
5, 132 (2000),
Application(s): ICC using mouse samples,
Abstract;
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