Product Details
| Alternative Name: | CALR, CRT |
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| Clone: | FMC 75 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant calreticulin. |
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| UniProt ID: | P27797 (human) |
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| Source: | Purified from ascites |
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| Species reactivity: | Human
Monkey
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| Applications: | ICC, IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Immunoprecipitation (12.5µg/ml)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Predicted MW of ~63kDa. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The multifunctional, multi -compartmental protein Calreticulin (Crt) functions as a soluble molecular chaperone of new or misfolded proteins, as well as a Ca2+-binding protein. Most abundant in the ER lumen, Crt expression also occurs in other membrane-bound organelles, the cell surface, and extracellularly. Also known as CRP-55, calregulin and HACBP (high affinity calcium-binding protein), Crt contains the ER-retrieval sequence, KDEL, and is the solub le paralog of the ER membrane protein Calnexin (Cnx). Crt's three domains include a 180 residue N-terminal domain, a proline-rich P domain residues 189 -288) that binds Ca2+ with high affinity and shares homology with Cnx and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity. The P-domain may interact with the co-chaperone ERp57 (Grp58), a thiol reductase. The NMR structure of the P -domain consists of an extended hairpin that appears to form a curved protrusion from the Crt core domain. Both Crt and its membrane bound homolog CNX interact with proteins and glycoproteins possessing monoglucosylated N -glycans. The Crt/Cnx cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligomerization, regulates ER degradation, and prevents incompletely folded glycoproteins from exiting to the Golgi complex. Crt also appears to function as an auto-antigen in systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis. A diversity of additional functions attributed to Crt includes adhesion, blood function, and cardiac and neuronal development gene expression. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Calreticulin: Lane 1: HeLa (Heat Shocked), Lane 2: Vero.
Immunohistochemistry analysis of human uterus tissue stained with Calreticulin, mAb (FMC 75) at 10µg/ml.
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Product Literature References
Tumor-targeted redox-regulating and antiangiogenic phototherapeutics nanoassemblies for self-boosting phototherapy: E. Jung, et al.; Biomaterials
298, 122127 (2023),
Abstract;
Conventional amphotericin B elicits markers of immunogenic cell death on leukemic blasts, mediates immunostimulatory effects on phagocytic cells, and synergizes with PD-L1 blockade: G. Kofla, et al.; Oncoimmunology
11, 2068109 (2022),
Abstract;
EDEM1 Regulates Amyloid Precursor Protein (APP) Metabolism and Amyloid-β Production: J. Nowakowska-Gołacka, et al.; Int. J. Mol. Sci.
23, 117 (2022),
Abstract;
PKHB1, a thrombospondin-1 peptide mimic, induces anti-tumor effect through immunogenic cell death induction in breast cancer cells: K.M.C. Rodríguez, et al.; Oncoimmunology
11, 2054305 (2022),
Abstract;
Production and Characterization of Peptide Antibodies to the C-Terminal of Frameshifted Calreticulin Associated with Myeloproliferative Diseases: F.P. Mughal, et al.; Int. J. Mol. Sci.
23, 6803 (2022),
Abstract;
Self-deliverable and self-immolative prodrug nanoassemblies as tumor targeted nanomedicine with triple cooperative anticancer actions: E. Jung, et al.; Biomaterials
287, 121681 (2022),
Abstract;
Two-Stage SN38 Release from a Core-Shell Nanoparticle Enhances Tumor Deposition and Antitumor Efficacy for Synergistic Combination with Immune Checkpoint Blockade: X. Jiang, et al.; ACS Nano
16, 21417 (2022),
Abstract;
Activation of plasmacytoid dendritic cells promotes AML-cell fratricide: K. Fatehchand, et al.; Oncotarget
12, 878 (2021),
Abstract;
Epitope Mapping of Monoclonal Antibodies to Calreticulin Reveals That Charged Amino Acids Are Essential for Antibody Binding: A.C. Bergmann, et al.; Antibodies
10, 31 (2021),
Abstract;
Polymer-ritonavir derivate nanomedicine with pH-sensitive activation possesses potent anti-tumor activity in vivo via inhibition of proteasome and STAT3 signaling: L. Sivák, et al.; J. Control Release
332, 563 (2021),
Application(s): Flow Cytometry on CT26 cells,
Abstract;
Calreticulin exploits TGF‐β for extracellular matrix induction engineering a tissue regenerative process: U.M. Pandya, et al.; FASEB J.
34, 15849 (2020),
Abstract;
Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death: L. Kasikova, et al.; Methods Enzymol.
632, 15 (2020),
Abstract;
A novel anti-HER2 anthracycline-based antibody-drug conjugate induces adaptive anti-tumor immunity and potentiates PD-1 blockade in breast cancer: L. D'Amico, et al.; J. Immunother. Cancer
7, 16 (2019),
Abstract;
Full Text
Caspase-11 mediates neutrophil chemotaxis and extracellular trap formation during acute gouty arthritis through alteration of cofilin phosphorylation: K. Caution, et al.; Front. Immunol.
10, 2519 (2019),
Abstract;
Complement Component C3 Is Highly Expressed in Human Pancreatic Islets and Prevents β Cell Death via ATG16L1 Interaction and Autophagy Regulation: B.C. King, et al.; Cell Metab.
29, 202 (2019),
Abstract;
The Biophysical Interaction of the Danger-Associated Molecular Pattern (DAMP) Calreticulin with the Pattern-Associated Molecular Pattern (PAMP) Lipopolysaccharide: U.M. Pandya, et al.; Int. J. Mol. Sci.
20, 408 (2019),
Abstract;
Full Text
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods
15, 909 (2018),
Abstract;
AAV-mediated gene delivery of the calreticulin anti-angiogenic domain inhibits ocular neovascularization: L. Tu, et al.; Angiogenesis
21, 95 (2018),
Abstract;
Targeting of drug-loaded nanoparticles to tumor sites increases cell death and release of danger signals: M. Alev, et al.; J. Control Release
285, 67 (2018),
Abstract;
Tumor-associated calreticulin variants functionally compromise the peptide loading complex and impair its recruitment of MHC-I: N. Arshad, et al.; J. Biol. Chem.
296, 9555 (2018),
Abstract;
Full Text
Gene Delivery of Calreticulin Anti-Angiogenic Domain Attenuates the Development of Choroidal Neovascularization in Rats: Y.S. Bee, et al.; Hum. Gene Ther.
5, 403 (2017),
Abstract;
Dendritic cells pulsed with tumor cells killed by high hydrostatic pressure induce strong immune responses and display therapeutic effects both in murine TC-1: R. Mikyskova, et al.; Int. J. Oncol.
48, 953 (2016),
Abstract;
Full Text
Cell density-induced changes in lipid composition and intracellular trafficking: S. Kavaliauskiene, et al.; Cell. Mol. Life Sci.
71, 1097 (2014),
Abstract;
Induction of the Unfolded Protein Response Drives Enhanced Metabolism and Chemoresistance in Glioma Cells: L.M. Epple, et al.; PLoS One
8, e73267 (2013),
Application(s): Immunohistochemistry using formalin-fixed, paraffin-embedded human glioma tumor or murine brain tissue samples and Western blot using human glioma tissue samples and cell lines,
Abstract;
Full Text
Proteinase 3, the Autoantigen in Granulomatosis with Polyangiitis, Associates with Calreticulin on Apoptotic Neutrophils, Impairs Macrophage Phagocytosis, and Promotes Inflammation: J. Gabillet, et al.; J. Immunol.
189, 2574 (2012),
Application(s): Flow cytometry using human neutrophils,
Abstract;
Full Text
Susceptibility of rhabdomyosarcoma cells to macrophage-mediated cytotoxicity: D. Herrmann, et al.; Oncoimmunology
1, 279 (2012),
Abstract;
Full Text
Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell
134, 769 (2008),
Application(s): WB using human cell lysates,
Abstract;
Expression of the molecular chaperone Hsp70 in detergent-resistant microdomains correlates with its membrane delivery and release: M. Bachelet, et al. ; J. Biol. Chem.
278, 21601 (2003),
Application(s): WB using human samples,
Abstract;
Regulation by heavy metals and temperature of the human BAG-3 gene, a modulator of Hsp70 activity: A. Leone, et al. ; FEBS Lett.
541, 15 (2003),
Application(s): ICC, WB using human samples,
Abstract;
Identification and pharmacological correction of a membrane trafficking defect associated with a mutation in the sulfonylurea receptor causing familial hyperinsulinism: A. Sivaprasadarao, et al. ; J. Biol. Chem.
276, 35947 (2001),
Application(s): ICC using human samples,
Abstract;
Pharmacological chaperones rescue cell-surface expression and function of misfolded V2 vasopressin receptor mutants: M. Bouvier, et al. ; J. Clin. Invest.
105, 887 (2000),
Application(s): ICC using monkey samples,
Abstract;
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