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DnaJ (E. coli) polyclonal antibody

 
ADI-SPA-410-D 50 µg 199.00 USD
 
ADI-SPA-410-F 200 µg 457.00 USD
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Product Details

Alternative Name:HSP40, Heat shock protein 40, Heat shock protein J
 
Host:Rabbit
 
Immunogen:E. coli DnaJ.
 
UniProt ID:P08622
 
Source:Purified from rabbit serum.
 
Species reactivity:E. coli
 
Applications:IP, WB
 
Recommended Dilutions/Conditions:Immunoprecipitation (1:200)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~41kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:DnaJ is a basic 41 kDa E. coli Heat shock protein which belongs to the molecular chaperone class of proteins. Bacterial DnaJ protein acts synergistically with the bacterial chaperone DnaK (Hsp70 homologue) and the other co-chaperone GrpE in suppressing eukaryotic and prokaryotic polypeptide aggregation, facilitating protein translocation through intracellular compartments or protein secretion, reactivating some partially aggregated enzymes, and activating pre-priming complex during initiation of DNA replication.
 
Regulatory Status:RUO - Research Use Only
 
DnaJ (E. coli) polyclonal antibody Western blot
Western blot analysis: Lane 1: DnaJ Recombinant E. coli Protein.
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DnaJ (E. coli) polyclonal antibody Western blot

Product Literature References

Overproduction of bacterial protein disulfide isomerase (DsbC) and its modulator (DsbD) markedly enhances periplasmic production of human nerve growth factor in Escherichia coli: T. Yura, et al. ; J. Biol. Chem. 276, 14393 (2001), Application(s): WB using bacteria (E. coli) samples, Abstract;
Hsp70 and hsp40 chaperones can inhibit self-assembly of polyglutamine proteins into amyloid-like fibrils: F.U. Hartl, et al. ; PNAS 97, 7841 (2000), Application(s): WB using bacteria (E. coli) samples, Abstract;

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