Product Details
| Alternative Name: | Chaperonin 10 |
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| Host: | Rabbit |
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| Immunogen: | E. coli GroES. |
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| UniProt ID: | P0A6F9 (strain K12) |
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| GenBank ID: | X07850 |
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| Source: | Whole rabbit serum. |
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| Species reactivity: | E. coli
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:5,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~10kDa by Western blot. |
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| Formulation: | Liquid. Whole rabbit serum. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | GroEL and GroES are E. coli chaperonins, which are homologs of mitochondrial chaperonins Hsp60 and Hsp10. GroEL is a double toriodal assembly of 14 identical subunits which form two heptameric rings stacked back-to-back, with a cavity at each end. GroEL and its co-chaperonin GroES facilitate protein folding with an ATP-dependent mechanism. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of GroES: Lane 1: MW marker, Lane 2: GroES Recombinant E. coli Protein, Lane 3: (Empty), Lane 4: E. coli Lysate.
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Product Literature References
Prolyl isomerases in a minimal cell - catalysis of protein folding by trigger factor from Mycoplasma genitalium: A. Pahl, et al. ; Eur. J. Biochem.
267, 3270 (2000),
Application(s): WB using bacteria (E. coli) samples,
Abstract;
Expression of a gene encoding a 16.9-kDa heat-shock protein, Oshsp16.9, in Escherichia coli enhances thermotolerance: C.Y. Lin, et al. ; PNAS
94, 10967 (1997),
Application(s): WB using bacteria (E. coli) samples,
Abstract;
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