Product Details
| Alternative Name: | Superoxide Dismutase, SOD2 |
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| MW: | ~25kDa |
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| Source: | Produced in E. coli. Mouse Mn SOD is fused at the N-terminus to a His-tag. |
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| UniProt ID: | P09671 |
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| Formulation: | Liquid. In 50mM TRIS-HCl, pH 7.5, containing 1.5 % glycerol and 80mM sodium chloride. |
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| Purity: | ≥90% (SDS-PAGE; Western blot) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Applications: | WB
Activity assay
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| Application Notes: | Activity Assay (superoxide dismutase). Western blot control. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide. There are three SOD isoenzymes in mammalian cells: extracellular SOD (EC SOD, SOD-3); copper and zinc-containing SOD (Cu/Zn SOD, SOD-1); and manganese-containing SOD (Mn SOD, SOD-2). Mn SOD is an 80 kDa tetramer that contains Mn ions and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from TNF-mediated apoptosis via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and modulation of the redox state of the mitochondria. Overexpression of this enzyme protects neurons from NMDA and nitric oxide-induced neurotoxicity. |
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| Regulatory Status: | RUO - Research Use Only |
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Western Blot analysis: Lane 1: MWM, Lane 2: Mouse Brain Tissue Extract, Lane 3: Recombinant Mn SOD Mouse Protein, probed with Mn SOD pAb (Prod. No. ADI-SOD-111).
SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 2µg, Lane 3: 5µg of Mn SOD (SOD2) Recombinant Mouse Protein.
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Product Literature References
Endurance training increases the efficiency of rat skeletal muscle mitochondria: J.A. Zoladz, et al.; Pflugers Arch.
468, 1709 (2016),
Application(s): Determination of protein levels through immunoblotting,
Abstract;
Full Text
Sod1 loss induces intrinsic superoxide accumulation leading to p53-mediated growth arrest and apoptosis: K. Watanabe, et al.; Int. J. Mol. Sci.
14, 10998 (2013),
Application(s): Western blot,
Abstract;
Full Text
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