Product Details
Alternative Name: | RELA |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to a portion of human p65 NF-κB. The sequence is completely conserved in mouse NF-κB p65. |
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UniProt ID: | Q04206 |
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GenBank ID: | M62399 |
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Source: | Purified from rabbit serum. |
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Species reactivity: | Human, Mouse, Rat Bovine, Dog, Guinea pig, Monkey, Porcine, Rabbit
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Applications: | WB
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Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~65kDa by Western blot. |
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Nuclear factor kappa-B (NFk) B) functions as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NF-kB is a heterodimer consisting of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA) (ref. 3), both of which exhibit sequence homology to the protooncogene c-Rel. p50 has an isoform called p49/p52, and both proteins derive from the amino-terminal of precursor protein p105 and p100, respectively. The p50/p65 heterodimer remains in inactive form in the cytosol as a complex with its inhibitor, IkB. Upon stimulation of cells by a variety of stimuli, including lipopolysaccharide (LPS), pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor, and viral infection, IkB becomes phosphorylated and degraded by the proteosome. The active NF-kB heterodimer translocates into the nucleus and induces gene expression. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Effect of EGCG on bronchial epithelial cell premalignant lesions induced by cigarette smoke and on its CYP1A1 expression: Q. Gu, et al.; Int. J. Mol. Med.
48, 220 (2021),
Abstract;
TNF Apoptosis Protection Fraction (TAPF) prevents apoptosis induced by TNF, but not by Fas or TRAIL, via NF-κB-induced increase in cFLIP: J.Y. Seol, et al.; Cytokine
75, 321 (2015),
Application(s): Western Blot,
Abstract;
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