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Sensitive - use less precious sample per test compared to Western blot
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Higher throughput - test up to 40 samples in duplicate in just less than 3 hours
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Quantitative - obtain statistically signficant results and compare data between multiple experiments
The HSP60 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
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Product Details
| Alternative Name: | Heat shock protein 60 |
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| Sensitivity: | 3.125 ng/ml (range 3.125 - 100 ng/ml) |
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| Assay Time: | <3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of HSP60 in cell lysates, serum, and tissue of human origin. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Crossreactivity: | HSP60 (100%). No reactivity with GroEL, HSP65, HSP27, HSP25, Cpn10, or GroES. |
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| Use/Stability: | Store all components at 4°C, except standard at -20°C. |
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| Shipping: | Dry Ice and Blue Ice |
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| Contents: | Microtiter plate, 5X Extraction reagent, Standard, Sample diluent, Wash buffer concentrate, Antibody, Antibody diluent, HRP Conjugate, Conjugate diluent, TMB Substrate, Stop solution 2 |
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| Scientific Background: | Hsp60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. Hsp60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of Hsp60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor. |
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| UniProt ID: | P10809 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Heat shock proteins (HSP 90, 70, 60, and 27) in Galleria mellonella (Lepidoptera) hemolymph are affected by infection with Conidiobolus coronatus (Entomophthorales): A.K. Wronska & M.I. Bogus; PLoS One
15, e0228556 (2020),
Abstract;
Full Text
Elevated heat shock proteins in bipolar disorder patients with hypothalamic pituitary adrenal axis dysfunction: Y. Cheng, et al.; Medicine
97, e11089 (2018),
Abstract;
Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese: A. Khadir, et al.; Front. Endocrinol. (Lausanne)
9, 16 (2018),
Abstract;
Full Text
Heat shock protein 70 and antibodies to heat shock protein 60 are associated with cerebrovascular atherosclerosis: R. Galovic, et al.; Clin. Biochem.
49, 66 (2016),
Application(s): Measurement of serum concentrations of Hsp60,
Abstract;
Influence of sex on cytokines, heat shock protein and oxidative stress markers in response to an acute total body resistance exercise protocol: R. Benini, et al.; J. Exerc. Sci. Fit.
13, 1 (2015),
Application(s): ELISA,
Abstract;
Full Text
Intrathecal heat shock protein 60 mediates neurodegeneration and demyelination in the CNS through a TLR4- and MyD88-dependent pathway: K. Rosenberger, et al.; Molec. Neurodegen.
10, 5 (2015),
Application(s): ELISA using mouse CSF,
Abstract;
Full Text
Oxidative stress markers at birth: Analyses of a neonatal population: M. Giuffrè, et al.; Acta Histochem.
117, 486 (2015),
Application(s): ELISA using human serum,
Abstract;
Elevated blood Hsp60, its structural similarities and cross-reactivity with thyroid molecules, and its presence on the plasma membrane of oncocytes point to the chaperonin as an immunopathogenic factor in Hashimoto's thyroiditis: A.M. Gammazza, et al.; Cell Stress Chaperones
19, 343 (2014),
Application(s): Human Plasma,
Abstract;
Full Text
Detection of autoantibodies against heat shock proteins and collapsin response mediator proteins in autoimmune retinopathy: G. Adamus, et al.; BMC Ophthalmol.
13, 48 (2013),
Application(s): ELISA using human serum samples,
Abstract;
Full Text
The small heat shock protein 27 is a key regulator of CD8+ CD57+ lymphocyte survival: K. Wood, et al.; J. Immunol.
15, 184 (2010),
Application(s): EIA using human lymphocytes,
Abstract;
Full Text
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