EGF receptor monoclonal antibody (6F1)



ADI-CSA-330-E	100 µg	372.00 USD
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Product Details

Alternative Name:	EGFR, epidermal growth factor receptor

Clone:	6F1

Host:	Mouse

Isotype:	IgG2b

Immunogen:	Recombinant human EGF receptor (C-terminal fragment).

UniProt ID:	P00533

Species reactivity:	Human

Applications:	Flow Cytometry, ICC, IP, WB

Recommended Dilutions/Conditions:	Flow cytometry: 1:100 (final concentration) Immunocytochemistry: 1:100 Immunoprecipitation: 2-5 µL/250 µL of cell extract from 2.5 x 10⁶ cells Western Blot: 1:1,000-1:2,000 for chemiluminescence detection system Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~170kDa by Western blot.

Purity Detail:	Ammonium sulfate precipitation purified.

Formulation:	Liquid. In PBS, pH 7.2 containing 50% glycerol.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	The epidermal growth factor receptor (EGFR) is a 170-kDa membrane bound receptor consisting of an extracellular ligand-binding domain, a single hydrophobic transmembrane region, and a tyrosine kinase domain-containing cytoplasmic region. Ligand binding induces receptor homo- or heterodimerization, triggering autophosphorylation of cytoplasmic tyrosine residues that provide docking sites for Src homology 2 (SH2) domain-containing signaling molecules. Src-mediated phosphorylation of EGFR at Tyr845 may contribute to EGFR hyperactivity in cancer, while autophosphorylation of EGFR at Tyr1068 and/or Tyr1173 in response to ligand binding is known to recruit cofactors (e.g. SHP1, RasGAP, SOS1, Grb2) that activate pathways associated with cell growth, apoptosis, adhesion, receptor endocytosis, and protein degradation.

Regulatory Status:	RUO - Research Use Only

EGF receptor monoclonal antibody (6F1) Western blot

Western blot analysis of human A431 cell lysate probed with EGFR mAb (6F1).

EGF receptor monoclonal antibody (6F1) Immunoprecipitation

Immunoprecipitation analysis of human EGF -R from A431 cells with 2µg (1) and 10μg (2) of EGFR mAb (6F1), and mouse IgG (3).

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Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;

MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation: S. Yoon, et al.; Immunity 47, 51 (2017), Abstract;

Differential regulation of epidermal growth factor receptor by hydrogen peroxide and flagellin in cultured lung alveolar epithelial cells: H. Nishi, et al.; Eur. J. Pharmacol. 748, 133 (2015), Application(s): Western blot, Abstract;

Phosphorylation of epidermal growth factor receptor at serine 1047 by MAP kinase-activated protein kinase-2 in cultured lung epithelial cells treated with flagellin: N. Noguchi, et al.; Arch. Biochem. Biophys. 529, 75 (2013), Application(s): Western blot, Abstract;

Selective cleavage of ErbB4 by G-protein-coupled gonadotropin-releasing hormone receptor in cultured hypothalamic neurons: S. Higa-Nakamine, et al.; J. Cell. Physiol. 227, 2492 (2012), Abstract;