- Higher throughput - homogeneous mix-and-read assay
- Flexible - signal generated can be read after 30 minutes or several hours
- Robust - Z' Factor >0.8
The PGE2 FPIA kit is a homogeneous mix-and-read fluorescence polarization enzyme immunoassay kit with. Results are obtained within just 30 minutes and the generated signal is stable for up to 20 hours. The PGE2 FPIA kit is ideal for high throughput screening (HTS) applications. A convenient homogeneous mix-and-read assay that is amendable to miniaturization and automation, this kit has a robust Z factor >0.8.
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Product Details
| Alternative Name: | Prostaglandin E2 |
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| Sensitivity: | 684 pg/ml (range 1,562 - 100,000 pg/ml) |
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| Assay Time: | 30 minutes |
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| Applications: | Fluorescence microscopy
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| Application Notes: | For the quantitative determination of PGE2 in culture supernatants from any species. |
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| Species reactivity: | Species independent
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| Use/Stability: | Store all components at 4°C, except standard and conjugate concentrate at -20°C. |
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| Shipping: | Blue Ice |
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| Contents: | Conjugate concentrate, Antibody, Assay buffer concentrate, Standard |
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| Scientific Background: | Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
A smartphone-based system for fluorescence polarization assays: Z. Zhao, et al.; Biosens. Bioelectron.
128, 91 (2019),
Abstract;
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