Product Details
| Alternative Name: | Tumor necrosis factor-α, TNFSF 2 |
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| Source: | Produced in E. coli. |
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| UniProt ID: | P01375 |
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| Formulation: | Lyophilized. Sterile filtered liquid, pH 7.2, containing 20mM PBS and 10mM sodium chloride. |
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| Purity: | ≥98% (SDS-PAGE; RP-HPLC) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Applications: | ELISA, WB
in vitro Assay
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| Application Notes: | Western blot control. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Upon reconstitution TNF-α should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
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| Scientific Background: | Tumor necrosis factor-alpha (TNFa), also known as cachectin, is a 17.5 kDa, 157 amino acid member of the TNF superfamily of cytokines that is a potent lymphoid factor with effects on a wide range of target cells. Active TNFa is produced as both soluble and membrane-anchored trimers by macrophages, NK cells, and T- and B-lymphocytes. TNF exerts proinflammatory signals via binding and inducing trimerization of TNF-receptor 1 (TNFR1) expressed on most normal and transformed cells, or to TNF-receptor 2 (TNFR2), expressed on endothelial and most immune cells. TNF signaling regulates hematopoiesis differentiation, endothelial cell activation, apoptosis, lipid metabolism, tumor progression, and immune surveillance, and dysregulation of TNF or its receptors is implicated in numerous disease states including cancer, osteoporosis, autoimmune diseases, diabetes, and atherosclerosis. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
CD40L membrane retention enhances the immunostimulatory effects of CD40 ligation: T. Elmetali, et al.; Sci. Rep.
10, 342 (2020),
Abstract;
Full Text
Effects of Immunosuppressive Medications on Mitochondrial Function: A. Nash, et al.; J. Surg. Res.
249, 50 (2020),
Abstract;
The unconventional myosin CRINKLED and its mammalian orthologue MYO7A regulate caspases in their signalling roles: M.H. Orme, et al.; Nat. Commun.
7, 10972 (2016),
Application(s): Western blot,
Abstract;
