[pTyr⁶⁹⁵/Tyr⁶⁹⁹]Stat5 A/B monoclonal antibody (5G4)

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ADI-905-673-100	100 µg	389.00 USD
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Alternative Name:	Signal transducer and activator of transcription 5A/B

Clone:	5G4

Host:	Mouse

Isotype:	IgG1

Immunogen:	Synthetic peptide corresponding to a portion of Stat5A/B phosphorylated at Tyr^695/699.

UniProt ID:	P42229 (STAT5A), P51692 (STAT5B)

Species reactivity:	Human

Applications:	ELISA, ICC, IHC, WB

Recommended Dilutions/Conditions:	ELISA (0.05µg/ml) Immunohistochemistry (1:50) Western Blot (0.5µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Purity Detail:	Thiophilic adsorption and size exclusion chromatography purified.

Formulation:	Liquid. In PBS containing 50% glycerol, PEG, sucrose, and 0.09% sodium azide.

Use/Stability:	Stable at -20°C up to 1 year.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	Stats (Signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, Stats enter the nucleus to regulate transcription of many different genes.

Regulatory Status:	RUO - Research Use Only

[pTyr695/Tyr699]Stat5 A/B monoclonal antibody (5G4) Western blot

Western blot analysis of untreated (1) and pervanadate treated (2) MCF-7 cells, probed with Stat5A/B (pTyr694/699) mAb (5G4).

[pTyr695/Tyr699]Stat5 A/B monoclonal antibody (5G4) Immunohistochemistry

Immunohistochemistry analysis of human hepatoma tissue with Stat5A/B (pTyr694/699) mAb (5G4).

[pTyr695/Tyr699]Stat5 A/B monoclonal antibody (5G4) Immunofluorescence

Immunofluorescent analysis (confocal) staining of MCF-7 cells using Stat5A/B (pTyr694/699) mAb (5G4) (green); nuclei are stained in blue pseudocolor using DRAQ5.

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ELISA, IHC, WB | Print as PDF

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