Product Details
| Alternative Name: | Receptor-interacting serine/threonine protein kinase 3, RIP-like protein kinase 3, Receptor-interacting protein 3 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to a portion of mouse RIP3. |
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| UniProt ID: | Q9QZL0 |
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| Species reactivity: | Mouse, Rat
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| Applications: | IHC (PS), WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 5µg/ml)
Western Blot (0.5-1.0µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~57kDa by Western blot. |
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| Purity Detail: | Peptide affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Scientific Background: | A member in the RIP kinase family was identified and designated RIP3 (Receptor-interacting serine/threonine protein kinase-3). RIP3 contains an N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNF-R1, mediates TNF-R1 induced apoptosis, and attenuates RIP and TNF-R1 induced NF-κB activation. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of RIP3 in mouse 3T3 whole cell lysate in the absence (A) or presence (B) of blocking peptide with RIP3 antibody at 1µg/ml.
Immunohistochemistry analysis of RIP3 in rat kidney tissue with RIP3 antibody at 5µg/ml.
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Product Literature References
Autophosphorylation at serine 166 regulates RIP kinase 1-mediated cell death and inflammation: L. Laurien, et al.; Nat. Commun.
11, 1747 (2020),
Abstract;
Full Text
Caspase-8, receptor-interacting protein kinase 1 (RIPK1), and RIPK3 regulate retinoic acid-induced cell differentiation and necroptosis: M. Someda, et al.; Cell Death Differ.
27, 1539 (2020),
Abstract;
Full Text
Gut Barrier Dysfunction Induced by Aggressive Fluid Resuscitation in Severe Acute Pancreatitis is Alleviated by Necroptosis Inhibition in Rats: Q.R. Cui, et al.; Shock
52, e107 (2019),
Abstract;
Glucocorticoid receptor dimers control intestinal STAT1 and TNF-induced inflammation in mice: M. Ballegeer, et al.; J. Clin. Invest.
128, 3265 (2018),
Abstract;
Full Text
LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis: N. Peltzer, et al.; Nature
557, 112 (2018),
Abstract;
Full Text
PELI1 Selectively Targets Kinase-Active RIP3 for Ubiquitylation-Dependent Proteasomal Degradation: S.W. Choi, et al.; Mol. Cell
70, 920 (2018),
Abstract;
Targeting intestinal epithelial cell–programmed necrosis alleviates tissue injury after intestinal ischemia/reperfusion in rats: X. Li, et al.; J. Surg. Res.
225, 117 (2018),
Abstract;
TNFR2 unlocks a RIPK1 kinase activity-dependent mode of proinflammatory TNFR1 signaling: D. Siegmund, et al.; Cell. Death. Dis.
9, 921 (2018),
Abstract;
Full Text
Caspase-1 Engagement and TLR-Induced c-FLIP Expression Suppress ASC/Caspase-8-Dependent Apoptosis by Inflammasome Sensors NLRP1b and NLRC5: N. Van Opdenbosch, et al.; Cell Rep.
21, 3427 (2017),
Abstract;
Full Text
Kinase-independent functions of RIPK1 regulate hepatocyte survival and liver carcinogenesis: T.M. Van, et al.; J. Clin. Invest.
127, 2662 (2017),
Abstract;
Full Text
Mitochondrial permeabilisation engages NF-κB dependent anti-tumour activity under caspase deficiency: E. Giampazolias, et al.; Nat. Cell Biol.
19, 1116 (2017),
Abstract;
Necroptosis is a key mediator of enterocytes loss in intestinal ischaemia/reperfusion injury: S. Wen, et al.; J. Cell. Mol. Med.
21, 432 (2017),
Abstract;
Full Text
The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death: E. Lafont, et al.; EMBO J.
36, 1147 (2017),
Abstract;
Full Text
Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria: A. Korwitz, et al.; J. Cell Biol.
212, 157 (2016),
Abstract;
Full Text
NEMO Prevents RIP Kinase 1-Mediated Epithelial Cell Death and Chronic Intestinal Inflammation by NF-κB-Dependent and -Independent Functions: K. Vlantis, et al.; Immunity
44, 553 (2016),
Application(s): Immunoblotting,
Abstract;
NEMO regulates a cell death switch in TNF signaling by inhibiting recruitment of RIPK3 to the cell death-inducing complex II: A. Pescatore, et al.; Cell Death Dis.
7, e2346 (2016),
Application(s): Cell stimulation and immunoprecipitation, MEF cells,
Abstract;
Full Text
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