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Convenient – user-friendly alternative to mass spectrometry
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Sensitive – measure as little as 0.78 ng/ml of 24(S)-hydroxycholesterol
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Time Saving – results from up to 36 samples in duplicate in just 2 hours
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Specific – low cross-reactivity with structurally related molecules
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Easy-to-use – liquid color-coded reagents reduce errors
The 24(S)-Hydroxycholesterol ELISA kit is a complete, colorimetric, competitive immunoassay kit for the quantitative determination of 24(S)-Hydroxycholesterol in tissue culture media (culture supernatant), cerebral spinal fluid and tissue homogenate samples with results in just 2 hours.
Detect normal levels of 24(S)-hydroxycholesterol. Normal human cerebral spinal fluid samples were diluted 1:2 in assay buffer and analyzed in the assay for 24(S)-Hydroxycholesterol levels. The blue bars represent the levels of 24(S)-Hydroxycholesterol in each sample as determined by ELISA. The dashed lines represent the maximum and minimum of the range of endogenous CSF 24(S)-Hydroxycholesterol levels as reported in the literature (Annu. Rev. Biochem. 2009.78:1017-1040.)
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Product Details
| Sensitivity: | 0.78 ng/ml (range 0.39-100ng/ml) |
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| Assay Time: | 2 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of species independent 24(S)-Hydroxycholesterol in cerebral spinal fluid, culture supernatants and tissue samples. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Species independent
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| Use/Stability: | All kit components should be stored at 4ºC. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | Microtiter Plate, Conjugate, Antibody, Assay Buffer, Wash Buffer Concentrate, Standard, TMB Substrate, Stop Solution 2, Streptavidin-HRP |
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| Scientific Background: | The homeostasis and trafficking of cholesterol is an essential component of both the central and peripheral nervous system in the maintenance of neuronal tissues, and disturbances in this homeostasis may be due to the onset of various neurological diseases such as Alzheimer’s Disease, Huntington’s Disease and multiple sclerosis. Apolipoprotein E and Cyp46 (also known as 24S-Cholesterol Hydroxylase) are both important in the homeostasis of cerebral cholesterol and thus are of clinical interest in understanding the relation of these molecules with the pathogenesis of these neurodegenerative diseases.
24-OHC, an enzymatically-generated side chain-hydroxylated derivative of cholesterol, is a pivotal marker in the study of cerebral cholesterol homeostasis. Cholesterol is unable to cross the blood-brain barrier however, Cyp46 enzyme converts cholesterol to the more soluble 24-OHC, and this hydroxylated form of cholesterol is able to cross the blood-brain barrier. This conversion allows for the reduction of cholesterol in the brain and the efflux of 24-OHC from the brain into cerebral spinal fluid and blood. The flux of 24-OHC has been seen in patients with a variety of neurodegenerative diseases. In the instance of Alzheimer’s disease, the change in 24S-hydroxycholesterol concentrations may be indicative of different pathogenetic mechanisms and/or the progression of the disease. As in the case of multiple sclerosis, concentrations of 24-OHC have been shown to decrease, likely due to the loss of neuronal cells responsible for the synthesis. |
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| Technical Info/Product Notes: |  |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Cholesterol metabolism is a druggable axis that independently regulates tau and amyloid-β in iPSC-derived Alzheimer's disease neurons: R. van der Kant, et al.; Cell Stem Cell
24, 363 (2019),
Application(s): 24(S)-hydroxycholesterol levels in culture supernatants,
Abstract;
Full Text
The technical reliability and biotemporal stability of cerebrospinal fluid biomarkers for profiling multiple pathophysiologies in Alzheimer’s disease: B.A. Trombetta, et al.; PLoS One
13, e0193707 (2018),
Abstract;
Full Text
Apolipoprotein D Overexpression Protects Against Kainate-Induced Neurotoxicity in Mice: O. Najyb, et al.; Mol. Neurobiol.
54, 3948 (2017),
Abstract;
Cholesterol overload induces apoptosis in SH-SY5Y human neuroblastoma cells through the up regulation of flotillin-2 in the lipid raft and the activation of BDNF/Trkb signaling: Y. Huang, et al.; Neuroscience
328, 201 (2016),
Application(s): 24-OHC quantification,
Abstract;
Necroptosis-like Neuronal Cell Death Caused by Cellular Cholesterol Accumulation: T. Funakoshi, et al.; J. Biol. Chem.
291, 25050 (2016),
Application(s): Determination of extracellular 24S hydroxycholesterol, SH-SY5Y human neuroblastoma cells,
Abstract;
Full Text
Neuronal cholesterol metabolism increases dendritic outgrowth and synaptic markers via a concerted action of GGTase-I and Trk: M. Moutinho, et al.; Sci. Rep.
6, 30928 (2016),
Application(s): 24(S)-hydroxycholesterol levels in culture supernatants,
Abstract;
Full Text
Neuronal DNA damage response-associated dysregulation of signalling pathways and cholesterol metabolism at the earliest stages of Alzheimer-type pathology: J.E. Simpson, et al.; Neuropath. Appl. Neurobiol.
42, 167 (2016),
Application(s): 24(S)-hydroxycholesterol levels in human cerebrospinal fluid,
Abstract;
A high-cholesterol diet enriched with polyphenols from Oriental plums (Prunus salicina) improves cognitive function and lowers brain cholesterol levels and neurodegenerative-related protein expression in mice: P.H. Kuo, et al.; Br. J. Nutr.
113, 1550 (2015),
Application(s): 24(S)-hydroxycholesterol levels in mouse brain extracts,
Abstract;
Plasma signature of neurological disease in the monogenetic disorder Niemann-Pick Type C: M.S. Alam, et al.; J. Biol. Chem.
289, 8051 (2014),
Application(s): ELISA using murine plasma,
Abstract;
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