[pSer¹⁵]HSP27 (human), ELISA kit

Only commercially available ELISA kit for ultra-sensitive detection of human phospho-Ser15 HSP27 in cell lysates, plasma, and serum.



ADI-900-170	96 wells	674.00 USD
Do you need bulk/larger quantities?

Ultra-sensitive and specific - be confident with your results and use less sample per test
Quantitative - obtain statistically significant results compared to semi-quantitative Western blot analysis
Higher throughput - assay up to 40 samples in duplicate in just 3 hours

The [pSer¹⁵]HSP27 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.

[pSer15]HSP27 (human), ELISA kit Standard curve

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Product Details

Alternative Name:	Heat shock protein 27

Sensitivity:	10.15 pg/ml (range 31.25 - 1000 pg/ml)

Assay Time:	3 hours

Applications:	ELISA, Colorimetric detection

Application Notes:	For the quantitative determination of human [pSer¹⁵]HSP27 in cell lysates, plasma, and serum.

Wavelength:	450 nm

Species reactivity:	Human

Crossreactivity:	Phopho HSP27 (Ser¹⁵) (100%), HSP27 (non-phospho) (0.885%), HSP70 (0.023%) and <0.016%: HSP25, HSP40, HSP90, αB Crystallin, αA Crystallin

Use/Stability:	Store all components at 4°C, except standard and antibody at -20°C.

Shipping:	Blue Ice

Contents:	Microtiter plate, Conjugate, Antibody, Assay buffer 27, Wash buffer concentrate, Standard, TMB Substrate, 5X Extraction reagent, Stop solution 2

Scientific Background:	Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-α, p70 S6K, PKD I, and PKC-δ. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.

UniProt ID:	P04792

GenBank ID:	L39370

Regulatory Status:	RUO - Research Use Only

Compatibility:	This product is compatible with the Absorbance 96 Plate Reader.