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Sensitive - measure as little as 3.3 pg/ml
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Reliable - fully validated in activated serum, plasma, and culture supernatants
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Simple - liquid color-coded reagents and pre-coated plates reduce error
The TGF-β1 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4 hours. Absorbance is read at 450 nm. This kit provides ultra-sensitive measurement of as little as 3.3 pg/mL TGF-β1 and has been fully validated in activated serum, plasma, and culture supernatants. The ready-to-use liquid color-coded reagents reduce error.
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Product Details
| Alternative Name: | Transforming growth factor β 1 |
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| Sensitivity: | 3.3 pg/ml in Assay Buffer 13; 10.8 pg/mL in Assay Buffer 30 (Range 31.25 - 1000 pg/ml) |
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| Assay Time: | 4 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of TGF-β1 in culture supernatants, plasma, and serum of human, mouse, rat, and cow origin. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human, Mouse, Rat
Bovine
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| Crossreactivity: | Human TGF-β1 (100%), TGF-β1.2 (2.23%), latent TGF-β1 (<0.71%) and <0.03%: TGF-β2, TGF-β3, LAP |
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| Use/Stability: | Store all components at 4°C, except standard at -20°C. |
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| Shipping: | Blue Ice |
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| Contents: | Microtiter plate, Conjugate, Antibody, Assay buffer 13, Assay buffer 30, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2 |
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| Scientific Background: | Transforming growth factor beta 1 is also known as TGFβ1, TGF β, and TGFB. The inactive form consists of a TGF-β1 homodimer that is non-covalently linked to a latency-associated peptide homodimer. The active form is a homodimer of mature TGF-β1 that is disulfide linked. The precursor is cleaved into mature TGF-β1 and the latency-associated peptide. Many cells can synthesize TGF-β1, which is secreted to function in proliferation, differentiation, transformation, signaling and apoptosis. This cytokine has been implicated in many diseases including diabetes, renal disease, chronic pulmonary obstructive disease, as well as many cancers including prostate, and colon. This protein is a potential tumor-specific cancer therapeutic. Specifically, as a stable soluble dimer, it selectively induces apoptosis in many transformed cells, but not in normal cells. The literature contains many reviews on the biology and function of TGF-β1. |
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| Technical Info/Product Notes: | Cited samples:
Cytokine ELISAs Cited Samples |
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| UniProt ID: | P01137 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Differential Immunometabolic Effects of High-Fat Diets Containing Coconut, Sunflower, and Extra Virgin Olive Oils in Female Mice: C.R. García, et al.; Mol. Nutr. Food Res.
66, e2200082 (2022),
Abstract;
Regulation of Cytokines and Dihydrotestosterone Production in Human Hair Follicle Papilla Cells by Supercritical Extraction-Residues Extract of Ulmus davidiana: Y.E. Kwon, et al.; Molecules
27, 1419 (2022),
Abstract;
Human platelet lysate as a replacement for fetal bovine serum in human corneal stromal keratocyte and fibroblast culture: N. Seidelmann, et al.; J. Cell. Mol. Med.
1111, 16912 (2021),
Abstract;
Inflammatory mediators resulting from transglutaminase 2 expressed in mast cells contribute to the development of Parkinson's disease in a mouse model: G.U. Hong, et al.; Toxicol. Appl. Pharmacol.
358, 10 (2018),
Application(s): ELISA using supernatants prepared from tissue homogenates,
Abstract;
Participation of arachidonic acid metabolism in the aortic aneurysm formation in patients with marfan syndrome: M.E. Soto, et al.; Front. Physiol.
9, 77 (2018),
Application(s): ELISA using tissue extracts,
Abstract;
Full Text
Substance P increases CCN2 dependent on TGF-beta yet Collagen Type I via TGF-beta1 dependent and independent pathways in tenocytes: N. Frara, et al.; Connect. Tissue Res.
59, 30 (2018),
Application(s): ELISA using culture supernatants,
Abstract;
Full Text
Thrombospondin-1 promotes matrix homeostasis by interacting with collagen and lysyl oxidase precursors and collagen cross-linking sites: S. Rosini, et al.; Sci. Signal.
11, eaar2566 (2018),
Application(s): ELISA using culture supernatants,
Abstract;
PPAR-α improves the recovery of lung function following acute respiratory distress syndrome by suppressing the level of TGF-β1: Y. Liu, et al.; Mol. Med. Rep.
16, 49 (2017),
Application(s): ELISA using human BALF, culture supernatants, and serum,
Abstract;
Full Text
Topical ocular delivery of TGF-β1 to the back of the eye: Implications in age-related neurodegenerative diseases: C.B.M. Platania, et al.; Int. J. Mol. Sci.
18, 2076 (2017),
Application(s): ELISA using filtrated liposomes,
Abstract;
Full Text
Improving osteoblast response in vitro by a nanostructured thin film with titanium carbide and titanium oxides clustered around graphitic carbon: G. Longo, et al.; PLoS One
11, e0152566 (2016),
Application(s): ELISA using cell lysates,
Abstract;
Full Text
Participation of oleic acid in the formation of the aortic aneurysm in Marfan syndrome patients: M.E. Soto, et al.; Prostaglandins Other Lipid Mediat.
123, 45 (2016),
Application(s): ELISA using human aortic homogenates,
Abstract;
Pirfenidone enhances the efficacy of combined radiation and sunitinib therapy: S.H. Choi, et al.; Biochem. Biophys. Res. Commun.
462, 138 (2015),
Application(s): ELISA using mouse culture medium,
Abstract;
The effect of IFN-γ and TGF-β in the functional activity of mononuclear cells in the presence of Entamoeba histolytica: L. Campelo Albuquerque Moraes, et al. ; Parasit. Vectors
8, 413 (2015),
Application(s): ELISA using culture supernatant of human MN cells,
Abstract;
Full Text
Angiotensin II-induced pro-fibrotic effects require p38MAPK activity and transforming growth factor beta 1 expression in skeletal muscle cells: M.G. Morales, et al.; Int. J. Biochem. Cell. Biol.
44, 1993 (2012),
Application(s): ELISA using culture supernatants,
Abstract;
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