Replaces Prod. #: ADI-900-130
- Sensitive - quantify down to 1 pg/mL
- Efficient - only 50 µl diluted sample required saving precious materials
- Specific - no cross-reactivity with related cytokines
- Results from up to 40 samples in duplicate in just 3 hours
- Ready-to-use reagents and pre-coated plates reduce errors and save your time
The IL-1β (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 450 nm. Quantify 1 pg/mL IL-1β with lot sample volume needed saving you precious sample.
Product Details
| Alternative Name: | Interleukin-1β |
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| Sensitivity: | 1 pg/mL |
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| Assay Time: | < 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of IL-1β in culture supernatant, plasma, serum, and urine of human origin. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Crossreactivity: | The following substances were tested and found to have no cross-reactivity: human IL-1α, IL-1ra, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, IFN-α, TGF-β, GM-CSF, G-CSF, OSM, MIP-1α, MIP-1β, LIF, MCP-1, G-CSF, RANTES, PF-4, βTG, GRO, IP-10, SCF, TNF-α, and TNF-β. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | +4°C |
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| Contents: | Standard, Standard Diluent Buffer, Coated 96-well plate, Conjugate, Streptavidin-HRP, Streptavidin-HRP Diluent, Wash Buffer Concentrate, TMB Solution, Stop Solution, Plate Sealers |
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| Scientific Background: | The interleukin-1 (IL-1) cytokine family consists of IL-1α, IL-1β, and IL-1Rα (receptor antagonist) which are expressed primarily by endothelial cells and activated macrophages. IL-1α and IL-1β elicit pro-inflammatory cytokine production and cellular activation, and are the primary mediators of hypothalamic regulation that results in fever. IL-1α and IL-1βsignals are transmitted via a dual subunit IL-1 receptor composed of IL-1R1 and the IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP is vital to IL-1 signaling, as it recruits the IL-1 receptor-associated kinase (IRAK) to the receptor complex, initiating NF-κB pathway activation. |
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| Technical Info/Product Notes: | Cited samples:
Cytokine ELISAs Cited Samples |
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| UniProt ID: | P01584 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Exploring association of melanoma-specific Bcl-xL with tumor immune microenvironment: A.M. Lucianò, et al.; J. Exp. Clin. Cancer Res.
42, 178 (2023),
Abstract;
Anti-inflammatory properties of S53P4 bioactive glass implant material: F.N. Barrak, et al.; J. Dent.
127, 104296 (2022),
Abstract;
Effects of photobiomodulation and a physical exercise program on the expression of inflammatory and cartilage degradation biomarkers and functional capacity in women with knee osteoarthritis: a randomized blinded study: P.G. Vassão, et al.; Adv. Rheumatol.
61, 62 (2021),
Abstract;
Involvement of the P2X7 receptor in the migration and metastasis of tamoxifen-resistant breast cancer: effects on small extracellular vesicles production: M. Park, et al.; Sci. Rep.
9, 11587 (2019),
Abstract;
Full Text
COMP-angiopoietin-1 ameliorates inflammation-induced lymphangiogenesis in dextran sulfate sodium (DSS)-induced colitis model: A. S. Lee, et al.; J. Mol. Med. (Berl.)
96, 459 (2018),
Application(s): EIA w. mouse serum,
Abstract;
Full Text
Follow-Up of Peripheral IL-1β and IL-6 and Relation with Apoptotic Death in Drug-Resistant Temporal Lobe Epilepsy Patients Submitted to Surgery: L. Lorigados Pedre, et al.; Behav. Sci. (Basel)
8, 21 (2018),
Abstract;
Gene therapy with RALA/iNOS composite nanoparticles significantly enhances survival in a model of metastatic prostate cancer: C.M. McCrudden, et al.; Cancer Nanotechnol.
9, 5 (2018),
Abstract;
Full Text
Cell phone use is associated with an inflammatory cytokine profile of parotid gland saliva: E.C. Siqueira, et al.; J. Oral Pathol. Med.
45, 682 (2016),
Application(s): ELISA using human saliva,
Abstract;
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci.
161, 37 (2016),
Application(s): Assessment of the inhibitory effect of VD2 on the production of pro-inflammatory cytokines,
Abstract;
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