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PGE₁ ELISA kit



ADI-900-005	96 wells	488.00 USD
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Results in from up to 38 samples in duplicate in less than 3 hours
Validated in multiple same matrices
Suitable for use with any species
Reproducible results day-after-day and lot-after-lot

The PGE₁ EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Assay up to 38 samples in duplicate per kit with excellent detection sensitivity.

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Product Details

Alternative Name:	Prostaglandin E₁

Sensitivity:	5.58 pg/ml (range 4.88 - 5,000 pg/ml)

Assay Time:	<3 hours

Applications:	ELISA, Colorimetric detection

Application Notes:	For the quantitative determination of PGE₁ in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample types include simulated interstitial lung fluid.

Wavelength:	405 nm

Species reactivity:	Species independent

Use/Stability:	Store all components 4°C, except conjugate -20°C.

Shipping:	Blue Ice

Contents:	DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution

Scientific Background:	Prostaglandin E₁ (PGE₁) is synthesized from dihomo-γ-linolenic acid (DGLA). PGE₁ has been shown to have a number of biological actions, including vasodilation, proliferation of vascular smooth muscle cells, platelet aggregation and has been shown to have insulin-like actions. Its effects are induced by receptor mediated elevation of cAMP. PGE₁ is the major prostaglandin in semen.

Regulatory Status:	RUO - Research Use Only

Compatibility:	This product is compatible with the Absorbance 96 Plate Reader.

Product Literature References

Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol. 12, 774086 (2022), Application(s): ELISA kit using hemolymph, Abstract; Full Text

Intermittent hypoxia alters dose dependent caffeine effects on renal prostanoids and receptors in neonatal rats: K.D. Beharry, et al.; Prostaglandins Other Lipid Mediat. 134, 57 (2018), Abstract;

Inhibitory effects of Aconiti Lateralis Radix Preparata on chronic intermittent cold-induced inflammation in the mouse hypothalamus: W. Kim, et al.; J. Ethnopharmacol. 215, 27 (2017), Abstract;

Effects of Almond- and Olive Oil-Based Docosahexaenoic- and Vitamin E-Enriched Beverage Dietary Supplementation on Inflammation Associated to Exercise and Age: X. Capo, et al.; Nutrients 8, 619 (2016), Application(s): Cytokine, Eicosanoids and Adhesion Molecules Determination, plasma, Abstract; Full Text

Enhanced Ca2+ response and stimulation of prostaglandin release by the bradykinin B2 receptor in human retinal pigment epithelial cells primed with proinflammatory cytokines: R. Catalioto, et al.; Biochem. Pharmacol. 97, 189 (2015), Application(s): ELISA using human cell culture supernatants, Abstract;

Influence of PEI as a core modifying agent on PLGA microspheres of PGE1, a pulmonary selective vasodilator: V. Gupta, et al.; Int. J. Pharm. 413, 51 (2011), Application(s): EIA using rat plasma and simulated interstitial lung fluid., Abstract; Full Text

PLGA microparticles encapsulating prostaglandin E1-hydroxypropyl-β-cyclodextrin (PGE1-HPβCD) complex for the treatment of pulmonary arterial hypertension (PAH): V. Gupta, et al.; Pharm. Res. 28, 1733 (2011), Abstract;